Team:KULeuven/14 August 2008

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Lab Work

Wet Lab

The PCR samples of the T7 polymerase with UmuD tag were put on gel. The results were not good. It is still a smear.

Some more ligation products were cut: [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] with XbaI and EcoRI; [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0062 R0062]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032] with SpeI and EcoRI. Only R0062+B0032 and J23100+B0032 were not properly cut. It is possible that the ligations were not correct.

The ligations that failed were set up again: [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] , [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015].

Dry Lab

Modeling

Nick transferred all his assets to us today. He delegated heavily and provided us with workable stuff for next week :) The wiki was also updated and remarks are all around, so a lot of work still to do.

Today, we implemented auto-activation of the Lux repressor, mimicking the original Vibrio Lux system. After a lot of messing about and a lot of long kinetic laws we finally managed it and it seems to be working correctly. It's very hard though to keep track of all the correct/changed parameters in such a large system :/

It's been said before this week, but things are looking quite good. We might have reached some sort of end point here where everything should be final. (hopefully)

Anyhow, time for a long weekend. See you on monday!

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Homepage code reformatted, and content added. Maarten made a image map of the team picture. IE fixes for tabs and picture gallery added.

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