Team:KULeuven/21 August 2008

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Contents

Lab Work

Wet Lab

Miniprep and digest of the following parts:

    • [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] (digest with EcoRI and XbaI) -> failed.
    • [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] and [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] (digest with SpeI and EcoRI) -> OK.

Electroporation of the following parts: [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_F1610 F1610] and [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2].

We did a PCR to test whether the transduced cells are correct (we want to see if they have the desired characteristics).

Dry Lab

General

Made a full-blown and fancy overview of the system (see below). All devices/composite parts have been redone as well and can be found in the Registry.

Superfinal.png

Modeling

Updated the CellDesigner model, we found out that the new version of CellDesigner can actually copy paste correctly, hooray! Tomorrow we might start re-testing and updating all the different devices/subcomponents, this will be stuff to put on the wiki.

We're also looking a bit at the stochastic solvers in MatLab, we'll see what we get. We might have to reshape a lot of kinetics to conform to the MassAction law shape.

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