Team:KULeuven/28 August 2008

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• The Team
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  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
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• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
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  GFP (LVA-tag) Other

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Lab Work

Gel 1: Test ligation with PCR. All 4 colonies of K145151+pSB1A2 ligated properly, but only the first colony of K145001+pSB1A2 ligated properly

Gel 2: Digests of B0015, C0012, C0060, K145015 and C0040

Wet Lab

The ligations with B0015 ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]) were electroporated again, but now they were plated on an ampicillin plate.

A glycerol stock was made of the succeeded ligations.

We ordered some new parts, because the modeling showed that a lot of things had to be changed. They arrived today and they were also plated.

The ligations of [http://partsregistry.org/Part:BBa_K145001 K145001] and [http://partsregistry.org/Part:BBa_K145151 K145151] with [http://partsregistry.org/Part:pSB1A2 pSB1A2] were miniprepped and then we did a PCR to test the ligation (primers VF/VR-2). See Gel 1.

The digests of [http://partsregistry.org/Part:BBa_R0011 R0011], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_C0062 C0062] and [http://partsregistry.org/Part:BBa_B0033 B0033] were put on gel and purified.

Some more digests were made, but this time we cut with SpeI, XbaI and PstI. We cut [http://partsregistry.org/Part:BBa_B0015 B0015] as insert (XbaI and PstI) and [http://partsregistry.org/Part:BBa_C0060 C0060], [http://partsregistry.org/Part:BBa_K145015 K145015], [http://partsregistry.org/Part:BBa_C0012 C0012] and [http://partsregistry.org/Part:BBa_C0040 C0040] were cut as vector (SpeI and PstI). See Gel 2.

Dry Lab

Ethics

We did some more research on bioethics and human practice.

Modeling

Great Succes: we succeeded in modeling cell-multiplication in a Matlab-environment. This will be our starting point for modeling cell division and work in a multi-cell environment! [we did what the lab couldn't do]

Parameter checking has almost reached its final state.

Simulations of the different parts has continued.

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