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From 2008.igem.org

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Things we did today

Wetlab work

Triparental conjugation

Grace

• Began triparental conjugation between DH5α transformed with pRL1383a, E. coli with RP1, and PCC6803 (see experiment)

• Innoculated BG-11 + 5% LB plates

Purified BBa_B0034 colonies

Krystle

• Streaked out colonies onto fresh LB+amp₁₀₀ plates

Preparation for making competent cells

Krystle & Grace

• Autoclaved detergent-free erlenmeyer flasks
• Innoculated 250 ml SOB w/ DH5α cryostock; shook overnight at 26C with shaking (150 rpm)

Made BG-11+sm+sp plates

Krystle

• Made 20 plates
• 2.5 μg/ml each of sm and sp, labeled Sm_2.5Sp_2.5

Made SOB media

Krystle

• 2 batches (and added antibiotic... oops! will autoclave again to get rid of it)

Made BG-11

Norman

• stored in room temp incubator bottom shelf
• will need to make bunch of 50 mL stock solution of TES and Thisulfate during some downtime.

Drylab Work

Sequence synthesis

Grace

• rbc promoter Biobrick sequence to be synthesized

Parts list

Norman

• Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper

Primer Design Review

Norman

• will review some primers tonight before shelling out money to buy'm

Discussion

Quote of the Day

• "We like lab math, except when we have to divide by even numbers. We can't divide by even numbers therefore we can't do binary fissions." -GK, KS
• "Bye" -GK, KS in synchronized tone...
  • "You girls should join a choir..." -GP
  • "You not only dress the same, you talk with the same voice at the same time, are you trying to confuse me again!?" -NW

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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