From 2008.igem.org

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Things we did today

Wetlab work

Plasmid prep of BB-pRL1383a

Grace

• E. coli did not grow. Reinoculated.

Extracted J04430 from filter paper

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded.

• Used 2 μl of filter paper solution for PCR to verify plasmid

Transformation

Grace

• Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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