From 2008.igem.org
Notebook
This year we are using a [http://www.google.com/notebook/public/05889908560840673300/BDRepIgoQp4H14akj Google Notebook] to co-ordinate our lab activities among team members. This includes our day to day activities and our progress.
Tentative Plans
Notes:
' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene
" denotes that the construct contians the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)
Construction Stages
1) Construction of CI"
2) Construction of Plac CI"
3) Ligate Plac (reverse) TT and Plac CI"
CI Repression Test
1) Construct Pconst CI?
2) Construct PR CI rfp"
3) Ligate above two constructs together (Pconst CI" PR CI rfp")
4) Test above repression by CI
Synthesize
1) Plac (reverse) TT
2) TT (reverse) PR
Lab Calendar
May
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| [http://2008.igem.org/wiki/index.php?title=Waterloo/1_May_2008&action=edit 1]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/2_May_2008&action=edit 2]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/3_May_2008&action=edit 3]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/4_May_2008&action=edit 4]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/5_May_2008&action=edit 5]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/6_May_2008&action=edit 6]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/7_May_2008&action=edit 7]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/8_May_2008&action=edit 8]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/9_May_2008&action=edit 9]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/10_May_2008&action=edit 10]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/11_May_2008&action=edit 11]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/12_May_2008&action=edit 12]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/13_May_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/14_May_2008&action=edit 14]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/15_May_2008&action=edit 15]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/16_May_2008&action=edit 16]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/17_May_2008&action=edit 17]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/18_May_2008&action=edit 18]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/19_May_2008&action=edit 19]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/20_May_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/21_May_2008&action=edit 21]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/22_May_2008&action=edit 22]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/23_May_2008&action=edit 23]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/24_May_2008&action=edit 24]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/25_May_2008&action=edit 25]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/26_May_2008&action=edit 26]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/27_May_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/28_May_2008&action=edit 28]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/29_May_2008&action=edit 29]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/30_May_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/31_May_2008&action=edit 31]
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June
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M | T | W | T | F | S | S
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| [http://2008.igem.org/Waterloo/1_June_2008 1]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/2_June_2008&action=edit 2]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/3_June_2008&action=edit 3]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/4_June_2008&action=edit 4]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/5_June_2008&action=edit 5]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/6_June_2008&action=edit 6]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/7_June_2008&action=edit 7]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/8_June_2008&action=edit 8]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/9_June_2008&action=edit 9]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/10_June_2008&action=edit 10]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/11_June_2008&action=edit 11]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/12_June_2008&action=edit 12]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/13_June_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/14_June_2008&action=edit 14]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/15_June_2008&action=edit 15]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/16_June_2008&action=edit 16]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/17_June_2008&action=edit 17]
| [http://2008.igem.org/Waterloo/18_June_2008 18]
| [http://2008.igem.org/Waterloo/19_June_2008 19]
| [http://2008.igem.org/Waterloo/20_June_2008 20]
| [http://2008.igem.org/Waterloo/21_June_2008 21]
| [http://2008.igem.org/Waterloo/22_June_2008 22]
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[http://2008.igem.org/Waterloo/23_June_2008 23]
| [http://2008.igem.org/Waterloo/24_June_2008 24]
| [http://2008.igem.org/Waterloo/25_June_2008 25]
| [http://2008.igem.org/Waterloo/26_June_2008 26]
| [http://2008.igem.org/Waterloo/27_June_2008 27]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/28_June_2008&action=edit 28]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/29_June_2008&action=edit 29]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/30_June_2008&action=edit 30]
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July
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M | T | W | T | F | S | S
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| [http://2008.igem.org/wiki/index.php?title=Waterloo/1_July_2008&action=edit 1]
| [http://2008.igem.org/Waterloo/2_July_2008 2]
| [http://2008.igem.org/Waterloo/3_July_2008 3]
| [http://2008.igem.org/Waterloo/4_July_2008 4]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/5_July_2008&action=edit 5]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/6_July_2008&action=edit 6]
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[http://2008.igem.org/Waterloo/7_July_2008 7]
| [http://2008.igem.org/Waterloo/8_July_2008 8]
| [http://2008.igem.org/Waterloo/9_July_2008 9]
| [http://2008.igem.org/Waterloo/10_July_2008 10]
| [http://2008.igem.org/Waterloo/11_July_2008 11]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/12_July_2008&action=edit 12]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/13_July_2008&action=edit 13]
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[http://2008.igem.org/Waterloo/14_July_2008 14]
| [http://2008.igem.org/Waterloo/15_July_2008 15]
| [http://2008.igem.org/Waterloo/16_July_2008 16]
| [http://2008.igem.org/Waterloo/17_July_2008 17]
| [http://2008.igem.org/Waterloo/18_July_2008 18]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/19_July_2008&action=edit 19]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/20_July_2008&action=edit 20]
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[http://2008.igem.org/Waterloo/21_July_2008 21]
| [http://2008.igem.org/Waterloo/22_July_2008 22]
| [http://2008.igem.org/Waterloo/23_July_2008 23]
| [http://2008.igem.org/Waterloo/24_July_2008 24]
| [http://2008.igem.org/Waterloo/25_July_2008 25]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/26_July_2008&action=edit 26]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/27_July_2008&action=edit 27]
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[http://2008.igem.org/Waterloo/28_July_2008 28]
| [http://2008.igem.org/Waterloo/29_July_2008 29]
| [http://2008.igem.org/Waterloo/30_July_2008 30]
| [http://2008.igem.org/Waterloo/31_July_2008 31]
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August
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M | T | W | T | F | S | S
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| [http://2008.igem.org/Waterloo/1_August_2008 1]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/2_August_2008&action=edit 2]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/3_August_2008&action=edit 3]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/4_August_2008&action=edit 4]
| [http://2008.igem.org/Waterloo/5_August_2008 5]
| [http://2008.igem.org/Waterloo/6_August_2008 6]
| [http://2008.igem.org/Waterloo/7_August_2008 7]
| [http://2008.igem.org/Waterloo/8_August_2008 8]
| [http://2008.igem.org/Waterloo/9_August_2008 9]
| [http://2008.igem.org/Waterloo/10_August_2008 10]
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[http://2008.igem.org/Waterloo/11_August_2008 11]
| [http://2008.igem.org/Waterloo/12_August_2008 12]
| [http://2008.igem.org/Waterloo/13_August_2008 13]
| [http://2008.igem.org/Waterloo/14_August_2008 14]
| [http://2008.igem.org/Waterloo/15_August_2008 15]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/16_August_2008&action=edit 16]
| [http://2008.igem.org/Waterloo/17_August_2008 17]
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[http://2008.igem.org/Waterloo/18_August_2008 18]
| [http://2008.igem.org/Waterloo/19_August_2008 19]
| [http://2008.igem.org/Waterloo/20_August_2008 20]
| [http://2008.igem.org/Waterloo/21_August_2008 21]
| [http://2008.igem.org/Waterloo/22_August_2008 22]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/23_August_2008&action=edit 23]
| [http://2008.igem.org/Waterloo/24_August_2008 24]
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[http://2008.igem.org/Waterloo/25_August_2008 25]
| [http://2008.igem.org/Waterloo/26_August_2008 26]
| [http://2008.igem.org/Waterloo/27_August_2008 27]
| [http://2008.igem.org/Waterloo/28_August_2008 28]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/29_August_2008&action=edit 29]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/30_August_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/31_August_2008&action=edit 31]
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September
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M | T | W | T | F | S | S
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[http://2008.igem.org/wiki/index.php?title=Waterloo/1_September_2008&action=edit 1]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/2_September_2008&action=edit 2]
| [http://2008.igem.org/Waterloo/3_September_2008 3]
| [http://2008.igem.org/Waterloo/4_September_2008 4]
| [http://2008.igem.org/Waterloo/5_September_2008 5]
| [http://2008.igem.org/Waterloo/6_September_2008 6]
| [http://2008.igem.org/Waterloo/7_September_2008 7]
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[http://2008.igem.org/Waterloo/8_September_2008 8]
| [http://2008.igem.org/Waterloo/9_September_2008 9]
| [http://2008.igem.org/Waterloo/10_September_2008 10]
| [http://2008.igem.org/Waterloo/11_September_2008 11]
| [http://2008.igem.org/Waterloo/12_September_2008 12]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/13_September_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/14_September_2008&action=edit 14]
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[http://2008.igem.org/Waterloo/15_September_2008 15]
| [http://2008.igem.org/Waterloo/16_September_2008 16]
| [http://2008.igem.org/Waterloo/17_September_2008 17]
| [http://2008.igem.org/Waterloo/18_September_2008 18]
| [http://2008.igem.org/Waterloo/19_September_2008 19]
| [http://2008.igem.org/Waterloo/20_September_2008 20]
| [http://2008.igem.org/Waterloo/21_September_2008 21]
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[http://2008.igem.org/Waterloo/22_September_2008 22]
| [http://2008.igem.org/Waterloo/23_September_2008 23]
| [http://2008.igem.org/Waterloo/24_September_2008 24]
| [http://2008.igem.org/Waterloo/25_September_2008 25]
| [http://2008.igem.org/Waterloo/26_September_2008 26]
| [http://2008.igem.org/Waterloo/27_September_2008 27]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/28_September_2008&action=edit 28]
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[http://2008.igem.org/Waterloo/29_September_2008 29]
| [http://2008.igem.org/Waterloo/30_September_2008 30]
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October
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M | T | W | T | F | S | S
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| [http://2008.igem.org/wiki/index.php?title=Waterloo/1_October_2008&action=edit 1]
| [http://2008.igem.org/Waterloo/2_October_2008 2]
| [http://2008.igem.org/Waterloo/3_October_2008 3]
| [http://2008.igem.org/Waterloo/4_October_2008 4]
| [http://2008.igem.org/Waterloo/5_October_2008 5]
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[http://2008.igem.org/Waterloo/6_October_2008 6]
| [http://2008.igem.org/Waterloo/7_October_2008 7]
| [http://2008.igem.org/Waterloo/8_October_2008 8]
| [http://2008.igem.org/Waterloo/9_October_2008 9]
| [http://2008.igem.org/Waterloo/10_October_2008 10]
| [http://2008.igem.org/Waterloo/11_October_2008 11]
| [http://2008.igem.org/Waterloo/12_October_2008 12]
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[http://2008.igem.org/Waterloo/13_October_2008 13]
| [http://2008.igem.org/Waterloo/14_October_2008 14]
| [http://2008.igem.org/Waterloo/15_October_2008 15]
| [http://2008.igem.org/Waterloo/16_October_2008 16]
| [http://2008.igem.org/Waterloo/17_October_2008 17]
| [http://2008.igem.org/Waterloo/18_October_2008 18]
| [http://2008.igem.org/Waterloo/19_October_2008 19]
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[http://2008.igem.org/Waterloo/20_October_2008 20]
| [http://2008.igem.org/Waterloo/21_October_2008 21]
| [http://2008.igem.org/Waterloo/22_October_2008 22]
| [http://2008.igem.org/Waterloo/23_October_2008 23]
| [http://2008.igem.org/Waterloo/24_October_2008 24]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/25_October_2008&action=edit 25]
| [http://2008.igem.org/Waterloo/26_October_2008 26]
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[http://2008.igem.org/Waterloo/27_October_2008 27]
| [http://2008.igem.org/Waterloo/28_October_2008 28]
| [http://2008.igem.org/Waterloo/29_October_2008 29]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/30_October_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/31_October_2008&action=edit 31]
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November
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M | T | W | T | F | S | S
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| [http://2008.igem.org/wiki/index.php?title=Waterloo/1_November_2008&action=edit 1]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/2_November_2008&action=edit 2]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/3_November_2008&action=edit 3]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/4_November_2008&action=edit 4]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/5_November_2008&action=edit 5]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/6_November_2008&action=edit 6]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/7_November_2008&action=edit 7]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/8_November_2008&action=edit 8]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/9_November_2008&action=edit 9]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/10_November_2008&action=edit 10]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/11_November_2008&action=edit 11]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/12_November_2008&action=edit 12]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/13_November_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/14_November_2008&action=edit 14]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/15_November_2008&action=edit 15]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/16_November_2008&action=edit 16]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/17_November_2008&action=edit 17]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/18_November_2008&action=edit 18]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/19_November_2008&action=edit 19]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/20_November_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/21_November_2008&action=edit 21]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/22_November_2008&action=edit 22]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/23_November_2008&action=edit 23]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/24_November_2008&action=edit 24]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/25_November_2008&action=edit 25]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/26_November_2008&action=edit 26]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/27_November_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/28_November_2008&action=edit 28]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/29_November_2008&action=edit 29]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/30_November_2008&action=edit 30]
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December
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M | T | W | T | F | S | S
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[http://2008.igem.org/wiki/index.php?title=Waterloo/1_December_2008&action=edit 1]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/2_December_2008&action=edit 2]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/3_December_2008&action=edit 3]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/4_December_2008&action=edit 4]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/5_December_2008&action=edit 5]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/6_December_2008&action=edit 6]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/7_December_2008&action=edit 7]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/8_December_2008&action=edit 8]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/9_December_2008&action=edit 9]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/10_December_2008&action=edit 10]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/11_December_2008&action=edit 11]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/12_December_2008&action=edit 12]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/13_December_2008&action=edit 13]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/14_December_2008&action=edit 14]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/15_December_2008&action=edit 15]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/16_December_2008&action=edit 16]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/17_December_2008&action=edit 17]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/18_December_2008&action=edit 18]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/19_December_2008&action=edit 19]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/20_December_2008&action=edit 20]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/21_December_2008&action=edit 21]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/22_December_2008&action=edit 22]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/23_December_2008&action=edit 23]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/24_December_2008&action=edit 24]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/25_December_2008&action=edit 25]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/26_December_2008&action=edit 26]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/27_December_2008&action=edit 27]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/28_December_2008&action=edit 28]
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[http://2008.igem.org/wiki/index.php?title=Waterloo/29_December_2008&action=edit 29]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/30_December_2008&action=edit 30]
| [http://2008.igem.org/wiki/index.php?title=Waterloo/31_December_2008&action=edit 31]
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Protocols
1-2-3- Mini prep
Recipes