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Team:UC Berkeley/LayeredAssembly
From 2008.igem.org
Layered Assembly
Picture of layered assembly File:Https://andersonlab.qb3.berkeley.edu/mediawiki/images/thumb/2/26/Assembly Layers.png/500px-Assembly Layers.png
In layered assembly, a part is first biobricked into an entry vector. The part is then passed into the appropiate assembly vector for 2 antibiotic assembly. A finished device may then be moved into a variety of destination vectors.
Motivation
The reactions required to perform each step of layered assembly can be outsourced to engineered E. coli. For example, to move a Biobrick part from an entry vector to an assembly vector usually requires digestion with the appropiate enzymes, gel purifying the correct fragment, then ligating the part with the assembly vector backbone. Using an E.coli with our Gateway device, this is reduced to just transforming the entry vector with the Biobrick part, lysing with the self-lysis device, and then using the lysate in a transformation. A protocol that cannot be easily automated is thus reduced to a series of liquid transfers and heating/cooling steps.
