Team:Hawaii/Notebook/2008-06-16

From 2008.igem.org

Revision as of 02:09, 17 June 2008 by Gracek (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Checked on plates in SC incubator

Grace
  • Checked on cell density and triparental conjugation plates
  • No cyanobacteria growth observed yet (they like to take their time). No contaminants observed.

Alkaline lysis prep

Grace & Krystle
  • Inoculated 5 ml LB+sp50 with 1 colony DH5α transformed w/ pRL1383a. Incubated overnight at 37C.

Restreaked DH5α w/ pRL1383a plate

Krystle
  • Restreaked on LB+sp100. Incubated at 37C overnight.

Drylab Work

Completed Biosafety Training

Grace & Krystle

Primers

Krystle
  • Edited primers for nir promoter

Alkaline lysis

Grace
  • Researched protocols for alkaline lysis plasmid prep. Plan on doing plasmid prep tomorrow.

Discussion

Quote of the Day

Krystle: Well, you can have some stuff.
Kevin (grad student): What stuff are you talking about?
Krystle: Um... I was going to say some gummi bears... but we ate them all.


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


Retrieved from "http://2008.igem.org/Team:Hawaii/Notebook/2008-06-16"