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Team:Chiba/protocol
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Revision as of 05:50, 26 October 2008 by
Aoi
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Notebook
Contents
1
Transformation
1.1
Zymo comp.
1.2
Electro poration
2
DNA Purification
2.1
Sigma prep
2.2
Zymo DNA Cleam&Concentrator Kit
3
Agarose gel electrophoresis
4
Digestion
5
PCR
6
Ligation
6.1
Gel extract
6.2
Dephosphorylation of DNA
6.3
Phosphorylation of DNA
6.4
Ligation
7
Time Delay Test
Transformation
Zymo comp.
Electro poration
DNA Purification
Sigma prep
Zymo DNA Cleam&Concentrator Kit
Agarose gel electrophoresis
Digestion
PCR
Ligation
Gel extract
Dephosphorylation of DNA
Phosphorylation of DNA
Ligation
Time Delay Test
"
