Team:UC Berkeley/GatewayPlasmid

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Gateway Plasmid Schemes

Scheme 1: Gateway Machinery in Assembly Vector

Two different variations of pBjh1601CA assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below).

PK112245.png

PK112246.png

The entry vector used in both cases was pBca1256 (see image below). PBca 1256.png

Scheme 2: Gateway Machinery in Entry Vector

PK112247.png
PK112248.png
PK112128.png

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