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Team:UC Berkeley/GatewayPlasmid
From 2008.igem.org
Gateway Plasmid Schemes
Scheme 1: Gateway Machinery in Assembly Vector
Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below).
The entry vector used in both cases was pBca1256 (see image below).
Scheme 2: Gateway Machinery in Entry Vector
The assembly vector used for each of the following entry vector variations is pK112128 (see image below.
Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} just before the attL1 site. These plasmids are named pK112247 and pK112248 respectively (see images below).
