Team:Rice University/Notebook/5 June 2008

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Thursday 5 June

  • Selim Sheikh:
    • Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
20080604 gel.jpg

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)










  • Taylor Stevenson
    • Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual.pdf.
    • Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.





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