Team:KULeuven/26 September 2008

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Lab Work

Wet Lab

We came to the lab for one day and we started with some digests (cut with EcoRI and XbaI): [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145270 K145270], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]. These digests were then put on gel and it looked very good. The bands were cut and extracted out of the gel.

With these digests we started some ligations:

(K145201)+(K145205) = [http://partsregistry.org/Part:BBa_K145280 K145280]
(K145201)+(R0040+E0240) = [http://partsregistry.org/Part:BBa_K145279 K145279]
(K145270)+(K145150+E0240) = [http://partsregistry.org/Part:BBa_K145267 K145267]
(R0040+B0033)+(C0056+B0015) = [http://partsregistry.org/Part:BBa_K145258 K145258]
(C0062)+(K145257) = [http://partsregistry.org/Part:BBa_K145258 K145258]

Dry Lab

We had another meeting today and we decided upon the things that still have to be done:

  • Send project title + abstract to iGEM HQ (Nick)
  • Presentation will be given by Stefanie, Jonas, Benjamien and Hanne
  • Presentation and poster will by made by Andim, Antoine, Dries and Jan
  • Check for T-shirts and sweaters to wear at the jamboree (Nick, Stefanie, Hanne)
  • Put an end evaluation on the wiki (Maarten)
  • Finishing ethics and human practice (Hanne and Benjamien)
  • Lab work, data analysis, documenting and sending parts to iGEM HQ (Nathalie, Elke and Stefanie)
  • Design primers to correct the parts that contain a wrong promoter
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