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Team:Waterloo/Notebook
From 2008.igem.org
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Contents |
Notebook
This year we are using a [http://www.google.com/notebook/public/05889908560840673300/BDRepIgoQp4H14akj Google Notebook] to co-ordinate our lab activities among team members. This includes our day to day activities and our progress.
Tentative Plans
Notes:
' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene
" denotes that the construct contains the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)
Construction Stages
1) Construction of CI"
2) Construction of Plac CI"
3) Ligate Plac (reverse) TT and Plac CI"
CI Repression Test
1) Construct Pconst CI?
2) Construct PR CI rfp"
3) Ligate above two constructs together (Pconst CI" PR CI rfp")
4) Test above repression by CI
Synthesize
1) Plac (reverse) TT
2) TT (reverse) PR
Daily Log
A log of our daily activities can be found on our Lab Calendar.
Freezer Management (BioMortar)
Our freezer management was done using [http://igem.uwaterloo.ca/biomortar BioMortar]. BioMortar is an application written in-house for administration of BioBricks. It understands standard assembly and can gather information from the Parts Registry. BioMortar will also capable of generating protocols for future projects.
Oligos
A list of all oligos we've used, either as PCR primers or for other purposes.
Protocols
The list of protocols used can be found on the Protocols page.
Recipes
Our recipes for making media can be found on the Recipes page.
