Team:KULeuven/6 September 2008

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Lab Work

Wet Lab

Busy day for a saturday ;)

  • Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts.
  • PCR with prefix-sufix primers on [http://partsregistry.org/Part:BBa_R1052 R1052] and the hybrid promoter. Gave some weird results. Will need to be looked at again.
  • Made a couple digests: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] were cut with EcoRI, E0040 was cut with SpeI and PstI, and [http://partsregistry.org/Part:BBa_B0015 B0015] was cut with XbaI and PstI. They were put on gel and then purified. The results looked very good.
  • Also made a digest of [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145205 K145205], [http://partsregistry.org/Part:BBa_K145253 K145253], [http://partsregistry.org/Part:BBa_K145251 K145251] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-)
  • Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015.
  • We set up a PCR to test our ligations using the VF2/VR primers.

Comic of the day

http://www.phdcomics.com/comics/archive/phd090308s.gif
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