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Team:Hawaii/Birds Eye View
{{Team:Hawaii/Common.css}} {{Team:Hawaii/Header}}

5 KB (626 words) - 03:27, 30 October 2008
Team:Hawaii/Project
{{Team:Hawaii/Header}} ===<onlyinclude>[[Team:Hawaii/Project/Part A|Part A]]: Mobilizable Broad-Host-Range Plasmid </onlyinclude

7 KB (1,011 words) - 03:46, 30 October 2008
Team:Hawaii/Construction of Broad-Host-Range Expression Vector
{{Team:Hawaii/Header}} ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR Amplification of pRL1383a]]===

5 KB (667 words) - 01:10, 16 August 2008
Team:Hawaii/Initial Synth. Oligo Assembly
==Protocol== ===[[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]===

19 KB (3,019 words) - 16:40, 25 July 2008
Team:Hawaii/PCR Amplification of pRL1383a
{{Team:Hawaii/Header}} * 1uL pRL1383a as template (1:10 dilution of [[Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli|large-scale plasmid prep]])

22 KB (3,349 words) - 00:48, 30 October 2008
Team:Hawaii/PCC6803 Electroporation of PCC6803
{{Team:Hawaii/Header}} **A [[Team:Hawaii/PCC6803 cell density| PCC6803 cell density]] test was performed and aligne

3 KB (464 words) - 18:33, 30 June 2008
Team:Hawaii/Notebook/2008-06- 2
{{Team:Hawaii/Header}} :* Protocol suggests 23C but growth was too slow. Also, protocol is for Mach10 cells, which grows much faster.

3 KB (445 words) - 02:35, 5 June 2008
Team:Hawaii/Notebook/2008-07-16
{{Team:Hawaii/Header}} :*pSB3K3 was restreaked [[Team:Hawaii/Notebook/2008-07-15|yesterday]], and today I am growing them up in LB media

1 KB (183 words) - 19:57, 17 July 2008
Team:Hawaii/Milestone
{{Team:Hawaii/Header}}[[Image:20080612-work breakdown structure.jpg|thumb|300px|right]] * Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)

2 KB (219 words) - 17:35, 15 August 2008
Team:Hawaii/Notebook/2008-07-22
{{Team:Hawaii/Header}} ...number. Used the protocol from Dr. Callahan's lab ([[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Protocols]]).

3 KB (438 words) - 00:40, 26 July 2008
Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli
{{Team:Hawaii/Header}} ...can be obtained by using CsCl/ethidium bromide equilibrium centrifugation (protocol found in Maniatis).

10 KB (1,527 words) - 08:31, 10 July 2008
Team:Hawaii/Meeting/2008-06-12
{{Team:Hawaii/Header}} '''Krystle: Signal Peptides''' [[Team:Hawaii/Project/Part_B | (see project proposal)]]

3 KB (536 words) - 21:46, 12 June 2008
Team:Hawaii/Test Competent E. Coli
...onential growth of E. coli seed stocks at 23C (Callahan cells, recommended protocol) result in higher competency than growth of E. coli at 37C (our cells)? ... cells were made on 7/11/08 with this [[Team:Hawaii/Make Competent E. Coli|protocol]].

4 KB (614 words) - 01:33, 16 July 2008
Team:Hawaii/Notebook/2008-06-17
{{Team:Hawaii/Header}} :* Same protocol as 6/3/08 competency test

1 KB (183 words) - 04:31, 18 June 2008
Team:Hawaii/Notebook/2008-06-20
{{Team:Hawaii/Header}} ===Electroporation Protocol===

705 B (98 words) - 06:51, 24 June 2008
Team:Hawaii/Notebook/2008-06-10
{{Team:Hawaii/Header}} ...r comparison of transformation efficiency with our competent cells. [[Team:Hawaii/Biobricks_extraction_%26_transformation_competency_test|(see experiment)]]

2 KB (225 words) - 01:06, 12 June 2008
Team:Hawaii/Notebook/2008-07- 9
{{Team:Hawaii/Header}} :* Followed protocol used for transformation of cells with BioBrick plasmids from filter

1 KB (167 words) - 03:41, 15 July 2008
Team:Hawaii/Meeting/2008-07-10
{{Team:Hawaii/Header}} # Oligo Annealing Ligation Protocol Updates(GK, KS)

766 B (106 words) - 08:18, 10 July 2008
Team:Hawaii/Notebook/2008-07-10
{{Team:Hawaii/Header}} ...) and annealed but not ligated products. Gel still had ladders. See [[Team:Hawaii/Initial_Synth._Oligo_Assembly|experiment]].

2 KB (273 words) - 09:10, 14 July 2008
Team:Hawaii/Project/Part B MaterialsMethodsResults
...es were created. The first followed ligation and restriction digest [[Team:Hawaii/Protocols |protocols]] using full concentrations of annealed products. The Following standard PCR protocol, [http://www.partsregistry.org/Part:BBa_E0040 GFP] was converted into the f

13 KB (1,867 words) - 03:04, 30 October 2008
Team:Hawaii/Meeting/2008-06-26
{{Team:Hawaii/Header}} ##* Primer Design notes (subtle variations & Hawaii protocol)

3 KB (431 words) - 02:35, 28 June 2008
Team:Hawaii/Biobrick conversions
==Protocol== ====[[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]]====

9 KB (1,260 words) - 20:53, 15 August 2008
Team:Hawaii/Biobricks extraction & transformation competency test
== Protocol == The same protocol as [[Team:Hawaii/Initial_ BioBrick_Plasmid_Extraction_From_Filter_Paper|"Initial BioBrick Pl

3 KB (433 words) - 02:40, 30 October 2008
Team:Hawaii/Ligation of pRL1383a Parts
{{Team:Hawaii/Header}} :*Protocol:

21 KB (3,321 words) - 03:32, 30 October 2008
Team:Hawaii/Notebook/2008-05-23
 var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");

1 KB (160 words) - 01:23, 12 June 2008
Team:Hawaii/Notebook/2008-05-24
 var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");

3 KB (398 words) - 01:28, 12 June 2008
Team:Hawaii/Notebook/2008-06- 1
{{Team:Hawaii/Header}} * Designed PCR protocol

320 B (45 words) - 04:39, 3 June 2008
Team:Hawaii/Notebook/2008-06-19
{{Team:Hawaii/Header}} :* Plasmid prep was performed according to the experimental protocol

1 KB (180 words) - 03:50, 26 June 2008
Team:Hawaii/Notebook/2008-06- 4
{{Team:Hawaii/Header}} :* made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media

1 KB (165 words) - 03:28, 12 June 2008
Team:Hawaii/Notebook/2008-06- 5
{{Team:Hawaii/Header}} :* Used same protocol as competency test on 6/3/08

923 B (132 words) - 03:27, 12 June 2008
Team:Hawaii/Notebook/2008-06-14
{{Team:Hawaii/Header}} :* Same protocol as 6/2 competent cells

1 KB (191 words) - 00:56, 15 June 2008
Team:Hawaii/Notebook/2008-08-26
{{Team:Hawaii/Header}} :*Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25

1 KB (187 words) - 03:31, 28 August 2008
Team:Hawaii/Meeting/2008-09- 8
{{Team:Hawaii/Header}} ## Gel cutting using SYBRSafe+Blue Light, [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Project:Phytoleum/Experiment/phaE_T855C_SigE_G855C_Bio

1 KB (171 words) - 11:12, 6 September 2008
Team:Hawaii/Conjugation Test: Comparing oriT with oriTr
{{Team:Hawaii/Header}} * The protocol was adapted from [[http://openwetware.org/wiki/Conjugation| openwetware.org

3 KB (451 words) - 05:53, 29 October 2008
Team:Hawaii/Initial BioBrick Plasmid Extraction From Filter Paper
==Protocol== Protocol adapted from Registry of Standard Biological Parts, [http://partsregistry.o

3 KB (443 words) - 07:18, 12 June 2008
Team:Hawaii/PCC6803 Cryostock Thaw Test
* Thaw PCC6803 -80C deep frozen stock to ensure that our cryogenic protocol works. This will ensure that we will have re animatable Synechocystis cell {{Team:Hawaii/Footer}}

2 KB (369 words) - 06:54, 12 June 2008