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Create the page "Team:Hawaii/Protocol" on this wiki!
- Team:Hawaii/Birds Eye View {{Team:Hawaii/Common.css}} {{Team:Hawaii/Header}}5 KB (626 words) - 03:27, 30 October 2008
- Team:Hawaii/Project {{Team:Hawaii/Header}} ===<onlyinclude>[[Team:Hawaii/Project/Part A|Part A]]: Mobilizable Broad-Host-Range Plasmid </onlyinclude7 KB (1,011 words) - 03:46, 30 October 2008
- Team:Hawaii/Construction of Broad-Host-Range Expression Vector {{Team:Hawaii/Header}} ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR Amplification of pRL1383a]]===5 KB (667 words) - 01:10, 16 August 2008
- Team:Hawaii/Initial Synth. Oligo Assembly ==Protocol== ===[[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]===19 KB (3,019 words) - 16:40, 25 July 2008
- Team:Hawaii/PCR Amplification of pRL1383a {{Team:Hawaii/Header}} * 1uL pRL1383a as template (1:10 dilution of [[Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli|large-scale plasmid prep]])22 KB (3,349 words) - 00:48, 30 October 2008
- Team:Hawaii/PCC6803 Electroporation of PCC6803 {{Team:Hawaii/Header}} **A [[Team:Hawaii/PCC6803 cell density| PCC6803 cell density]] test was performed and aligne3 KB (464 words) - 18:33, 30 June 2008
- Team:Hawaii/Notebook/2008-06- 2 {{Team:Hawaii/Header}} :* Protocol suggests 23C but growth was too slow. Also, protocol is for Mach10 cells, which grows much faster.3 KB (445 words) - 02:35, 5 June 2008
- Team:Hawaii/Notebook/2008-07-16 {{Team:Hawaii/Header}} :*pSB3K3 was restreaked [[Team:Hawaii/Notebook/2008-07-15|yesterday]], and today I am growing them up in LB media1 KB (183 words) - 19:57, 17 July 2008
- Team:Hawaii/Milestone {{Team:Hawaii/Header}}[[Image:20080612-work breakdown structure.jpg|thumb|300px|right]] * Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)2 KB (219 words) - 17:35, 15 August 2008
- Team:Hawaii/Notebook/2008-07-22 {{Team:Hawaii/Header}} ...number. Used the protocol from Dr. Callahan's lab ([[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Protocols]]).3 KB (438 words) - 00:40, 26 July 2008
- Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli {{Team:Hawaii/Header}} ...can be obtained by using CsCl/ethidium bromide equilibrium centrifugation (protocol found in Maniatis).10 KB (1,527 words) - 08:31, 10 July 2008
- Team:Hawaii/Meeting/2008-06-12 {{Team:Hawaii/Header}} '''Krystle: Signal Peptides''' [[Team:Hawaii/Project/Part_B | (see project proposal)]]3 KB (536 words) - 21:46, 12 June 2008
- Team:Hawaii/Test Competent E. Coli ...onential growth of E. coli seed stocks at 23C (Callahan cells, recommended protocol) result in higher competency than growth of E. coli at 37C (our cells)? ... cells were made on 7/11/08 with this [[Team:Hawaii/Make Competent E. Coli|protocol]].4 KB (614 words) - 01:33, 16 July 2008
- Team:Hawaii/Notebook/2008-06-17 {{Team:Hawaii/Header}} :* Same protocol as 6/3/08 competency test1 KB (183 words) - 04:31, 18 June 2008
- Team:Hawaii/Notebook/2008-06-20 {{Team:Hawaii/Header}} ===Electroporation Protocol===705 B (98 words) - 06:51, 24 June 2008
- Team:Hawaii/Notebook/2008-06-10 {{Team:Hawaii/Header}} ...r comparison of transformation efficiency with our competent cells. [[Team:Hawaii/Biobricks_extraction_%26_transformation_competency_test|(see experiment)]]2 KB (225 words) - 01:06, 12 June 2008
- Team:Hawaii/Notebook/2008-07- 9 {{Team:Hawaii/Header}} :* Followed protocol used for transformation of cells with BioBrick plasmids from filter1 KB (167 words) - 03:41, 15 July 2008
- Team:Hawaii/Meeting/2008-07-10 {{Team:Hawaii/Header}} # Oligo Annealing Ligation Protocol Updates(GK, KS)766 B (106 words) - 08:18, 10 July 2008
- Team:Hawaii/Notebook/2008-07-10 {{Team:Hawaii/Header}} ...) and annealed but not ligated products. Gel still had ladders. See [[Team:Hawaii/Initial_Synth._Oligo_Assembly|experiment]].2 KB (273 words) - 09:10, 14 July 2008
- Team:Hawaii/Project/Part B MaterialsMethodsResults ...es were created. The first followed ligation and restriction digest [[Team:Hawaii/Protocols |protocols]] using full concentrations of annealed products. The Following standard PCR protocol, [http://www.partsregistry.org/Part:BBa_E0040 GFP] was converted into the f13 KB (1,867 words) - 03:04, 30 October 2008
- Team:Hawaii/Meeting/2008-06-26 {{Team:Hawaii/Header}} ##* Primer Design notes (subtle variations & Hawaii protocol)3 KB (431 words) - 02:35, 28 June 2008
- Team:Hawaii/Biobrick conversions ==Protocol== ====[[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]]====9 KB (1,260 words) - 20:53, 15 August 2008
- Team:Hawaii/Biobricks extraction & transformation competency test == Protocol == The same protocol as [[Team:Hawaii/Initial_ BioBrick_Plasmid_Extraction_From_Filter_Paper|"Initial BioBrick Pl3 KB (433 words) - 02:40, 30 October 2008
- Team:Hawaii/Ligation of pRL1383a Parts {{Team:Hawaii/Header}} :*Protocol:21 KB (3,321 words) - 03:32, 30 October 2008
- Team:Hawaii/Notebook/2008-05-23 <!-- Team Hawaii Tracker --> var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");1 KB (160 words) - 01:23, 12 June 2008
- Team:Hawaii/Notebook/2008-05-24 <!-- Team Hawaii Tracker --> var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");3 KB (398 words) - 01:28, 12 June 2008
- Team:Hawaii/Notebook/2008-06- 1 {{Team:Hawaii/Header}} * Designed PCR protocol320 B (45 words) - 04:39, 3 June 2008
- Team:Hawaii/Notebook/2008-06-19 {{Team:Hawaii/Header}} :* Plasmid prep was performed according to the experimental protocol1 KB (180 words) - 03:50, 26 June 2008
- Team:Hawaii/Notebook/2008-06- 4 {{Team:Hawaii/Header}} :* made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media1 KB (165 words) - 03:28, 12 June 2008
- Team:Hawaii/Notebook/2008-06- 5 {{Team:Hawaii/Header}} :* Used same protocol as competency test on 6/3/08923 B (132 words) - 03:27, 12 June 2008
- Team:Hawaii/Notebook/2008-06-14 {{Team:Hawaii/Header}} :* Same protocol as 6/2 competent cells1 KB (191 words) - 00:56, 15 June 2008
- Team:Hawaii/Notebook/2008-08-26 {{Team:Hawaii/Header}} :*Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/251 KB (187 words) - 03:31, 28 August 2008
- Team:Hawaii/Meeting/2008-09- 8 {{Team:Hawaii/Header}} ## Gel cutting using SYBRSafe+Blue Light, [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Project:Phytoleum/Experiment/phaE_T855C_SigE_G855C_Bio1 KB (171 words) - 11:12, 6 September 2008
- Team:Hawaii/Conjugation Test: Comparing oriT with oriTr {{Team:Hawaii/Header}} * The protocol was adapted from [[http://openwetware.org/wiki/Conjugation| openwetware.org3 KB (451 words) - 05:53, 29 October 2008
- Team:Hawaii/Initial BioBrick Plasmid Extraction From Filter Paper ==Protocol== Protocol adapted from Registry of Standard Biological Parts, [http://partsregistry.o3 KB (443 words) - 07:18, 12 June 2008
- Team:Hawaii/PCC6803 Cryostock Thaw Test * Thaw PCC6803 -80C deep frozen stock to ensure that our cryogenic protocol works. This will ensure that we will have re animatable Synechocystis cell {{Team:Hawaii/Footer}}2 KB (369 words) - 06:54, 12 June 2008
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