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Team:Bologna/Notebook
From 2008.igem.org
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| '''Protocol''' | | '''Protocol''' | ||
| '''Safety level''' | | '''Safety level''' | ||
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| [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]] | | [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Amplification|Amplification]] | | [[Team:Bologna/Protocols#Amplification|Amplification]] | ||
| 1 | | 1 | ||
| - | |- | + | |- |
| [[Team:Bologna/Protocols#Transformation|Transformation]] | | [[Team:Bologna/Protocols#Transformation|Transformation]] | ||
| 1 | | 1 | ||
| - | |- | + | |- |
| [[Team:Bologna/Protocols#Inoculation|Inoculation]] | | [[Team:Bologna/Protocols#Inoculation|Inoculation]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Miniprep|Miniprep]] | | [[Team:Bologna/Protocols#Miniprep|Miniprep]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Digestion_reaction|Digestion reaction]] | | [[Team:Bologna/Protocols#Digestion_reaction|Digestion reaction]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Gel_preparation|Gel preparation]] | | [[Team:Bologna/Protocols#Gel_preparation|Gel preparation]] | ||
| 2 | | 2 | ||
| - | |- | + | |- |
| [[Team:Bologna/Protocols#Electrophoretic_run|Electrophoretic run]] | | [[Team:Bologna/Protocols#Electrophoretic_run|Electrophoretic run]] | ||
| 2 | | 2 | ||
| - | |- | + | |- |
| [[Team:Bologna/Protocols#Gel_extraction|Gel extraction]] | | [[Team:Bologna/Protocols#Gel_extraction|Gel extraction]] | ||
| 2 | | 2 | ||
| - | |- | + | |- |
| [[Team:Bologna/Protocols#Ligation_reaction|Ligation reaction]] | | [[Team:Bologna/Protocols#Ligation_reaction|Ligation reaction]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Chemiocompetent_cells|Chemiocompetent cells]] | | [[Team:Bologna/Protocols#Chemiocompetent_cells|Chemiocompetent cells]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Mediums_and_buffers|Mediums and buffers]] | | [[Team:Bologna/Protocols#Mediums_and_buffers|Mediums and buffers]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Antibiotics_stocks_preparation|Antibiotics stocks preparation]] | | [[Team:Bologna/Protocols#Antibiotics_stocks_preparation|Antibiotics stocks preparation]] | ||
| 1 | | 1 | ||
| - | |- | + | |- |
| [[Team:Bologna/Protocols#IPTG_stocks_preparation|IPTG stocks preparation]] | | [[Team:Bologna/Protocols#IPTG_stocks_preparation|IPTG stocks preparation]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#Fluorescence_test|Fluorescence test]] | | [[Team:Bologna/Protocols#Fluorescence_test|Fluorescence test]] | ||
| 1 | | 1 | ||
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| [[Team:Bologna/Protocols#M9_supplemented_media|M9 supplemented media]] | | [[Team:Bologna/Protocols#M9_supplemented_media|M9 supplemented media]] | ||
| 1 | | 1 | ||
|} | |} | ||
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= Week 1: from 07/21/08 to 07/27/08 = | = Week 1: from 07/21/08 to 07/27/08 = | ||
Revision as of 13:51, 9 October 2008
| HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK | BIOSAFETY |
|---|
Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Contents |
Protocols
Week 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
Week 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
Week 3: from 08/04/08 to 08/10/08
