Team:Bologna/Notebook

From 2008.igem.org

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Revision as of 14:44, 9 October 2008

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HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK BIOSAFETY


Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

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Contents

Protocols

SafetyLevels.jpg
Protocol Safety level
Plates preparation
1
Amplification
1
Transformation
1
Inoculation
1
Miniprep
1
Digestion reaction
1
Gel preparation
2
Electrophoretic run
2
Gel extraction
2
Ligation reaction
1
Chemiocompetent cells
1
Mediums and buffers
1
Antibiotics stocks preparation
1
IPTG stocks preparation
1
Fluorescence test
1
M9 supplemented media
1


Week 1: from 07/21/08 to 07/27/08

  1. Enzymatic digestion of: C0051 E\S, LACI* X\P.
  2. Gel preparation , electrophoretic run.
  3. Amplification of: TETR , PUV, PRECA.


Week 2: from 07/28/08 to 08/03/08

  1. Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
  2. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240


Week 3: from 08/04/08 to 08/10/08



















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