Team:Bologna/Notebook
From 2008.igem.org
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- | [[Image:SafetyLevels.jpg|thumb|500px|For more information, see [[Biosafety page]]]] | + | [[Image:SafetyLevels.jpg|thumb|500px|For more information, see [[Team:Bologna/Biosafety|Biosafety page]]]] |
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Revision as of 14:50, 9 October 2008
HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK | BIOSAFETY |
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Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Contents |
Protocols
Protocol | Biosafety level |
Plates preparation | |
Amplification | |
Transformation | |
Inoculation | |
Miniprep | |
Digestion reaction | |
Gel preparation | |
Electrophoretic run | |
Gel extraction | |
Ligation reaction | |
Chemiocompetent cells | |
Mediums and buffers | |
Antibiotics stocks preparation | |
IPTG stocks preparation | |
Fluorescence test | |
M9 supplemented media | |
Week 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
Week 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
Week 3: from 08/04/08 to 08/10/08