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Team:Bologna/Notebook
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Revision as of 17:21, 10 October 2008 by Rita.morini (Talk | contribs)
| HOME | THE PROJECT | THE TEAM | PARTS SUBMITTED TO THE REGISTRY | MODELING | NOTEBOOK | BIOSAFETY |
|---|
Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Contents |
Protocols
| Protocol | Biosafety level |
| Plates preparation | |
| Amplification | |
| Transformation | |
| Inoculation | |
| Miniprep | |
| Digestion reaction | |
| Gel preparation | |
| Electrophoretic run | |
| Gel extraction | |
| Ligation reaction | |
| Chemiocompetent cells | |
| Mediums and buffers | |
| Antibiotics stocks preparation | |
| IPTG stocks preparation | |
| Fluorescence test | |
| M9 supplemented media | |
Week 1: from 07/21/08 to 07/27/08
- Enzymatic digestion of: C0051 E\S, LACI* X\P.
- Gel preparation , electrophoretic run.
- Amplification of: TETR , PUV, PRECA.
Week 2: from 07/28/08 to 08/03/08
- Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
- Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240
Week 3: from 08/04/08 to 08/10/08
