Team:Caltech/Protocols/MIC Assay
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- | + | # Grow one 5 ml culture to saturation at 37C with shaking in the indicated media and antibiotics as necessary. | |
+ | # Back dilute parent culture 1:50 into 5 ml of the same media and antibiotics plus differing amounts of hydrogen peroxide and let shake at 37C overnight. | ||
+ | # Record MIC as the concentration of hydrogen peroxide necessary to prevent overnight turbid growth. | ||
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Latest revision as of 00:14, 30 October 2008
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Measuring Minimum Inhibitory Concentration
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