Team:Chiba/Calendar-Home/26 August 2008
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(New page: >Home | Notebook 25 August 2008 <|> 27 August 2008 ==Labora...) |
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Line 18: | Line 18: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Sample DNA</td> | + | <td>Sample DNA(μL)</td> |
<td>30</td> | <td>30</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Loading Dye</td> | + | <td>Loading Dye(μL)</td> |
<td>6</td> | <td>6</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>36</td> | <td>36</td> | ||
</tr> | </tr> | ||
Line 68: | Line 68: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Sample DNA</td> | + | <td>Sample DNA(μL)</td> |
<td>2</td> | <td>2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Loading Dye</td> | + | <td>Loading Dye(μL)</td> |
<td>2</td></tr> | <td>2</td></tr> | ||
<tr> | <tr> | ||
- | <td> | + | <td>H<sub>2</sub>O(μL)</td> |
<td>8</td></tr> | <td>8</td></tr> | ||
<tr> | <tr> | ||
- | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>12</td></tr> | <td>12</td></tr> | ||
</table> | </table> | ||
Line 96: | Line 96: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Sample DNA</td> | + | <td>Sample DNA(μL)</td> |
<td>26</td> | <td>26</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>SAP</td> | + | <td>SAP(μL)</td> |
<td>1</td></tr> | <td>1</td></tr> | ||
<tr> | <tr> | ||
- | <td>SAP Buffer</td> | + | <td>SAP Buffer(μL)</td> |
<td>3</td></tr> | <td>3</td></tr> | ||
<tr> | <tr> | ||
- | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
- | <td> | + | <td>30</td></tr> |
</table> | </table> | ||
Line 126: | Line 126: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Loading Dye</td> | + | <td>Loading Dye(μL)</td> |
<td>1</td> | <td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>4</td> | <td>4</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>6</td></tr> | <td>6</td></tr> | ||
</table> | </table> | ||
Line 148: | Line 148: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>insert①C0178</td> | + | <td>insert①C0178(μL)</td> |
<td>1</td><td>-</td><td>-</td><td>1</td><td>-</td> | <td>1</td><td>-</td><td>-</td><td>1</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>insert②C0170</td> | + | <td>insert②C0170(μL)</td> |
<td>-</td><td>1</td><td>-</td><td>-</td><td>1</td> | <td>-</td><td>1</td><td>-</td><td>-</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>vector③J04500</td> | + | <td>vector③J04500(μL)</td> |
<td>-</td><td>-</td><td>2</td><td>2</td><td>2</td> | <td>-</td><td>-</td><td>2</td><td>2</td><td>2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>ligase</td> | + | <td>ligase(μL)</td> |
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Buffer</td> | + | <td>Buffer(μL)</td> |
<td>2</td><td>2</td><td>2</td><td>2</td><td>2</td> | <td>2</td><td>2</td><td>2</td><td>2</td><td>2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>dH<sub>2</sub>O</td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>6</td><td>6</td><td>5</td><td>4</td><td>4</td> | <td>6</td><td>6</td><td>5</td><td>4</td><td>4</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
</tr> | </tr> | ||
Line 184: | Line 184: | ||
::#insert①C0178+vector③J04500 | ::#insert①C0178+vector③J04500 | ||
::#insert②C0170+vector③J04500 | ::#insert②C0170+vector③J04500 | ||
- | |||
- | |||
===Team:Output=== | ===Team:Output=== |
Revision as of 10:14, 24 October 2008
25 August 2008 <|> 27 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- (8/25)--->Gel Check
|
|
|
--->Gel Extract
- --->Zymo Clean
- 5μl
- 5μl
- 27μl
- --->Gel Check
|
- --->SAP
Sample No. 3 Sample DNA(μL) 26 SAP(μL) 1 SAP Buffer(μL) 3 TOTAL(μL) 30
- --->Zymo Clean
- Sample No.3 -->9μl
- --->Gel Check
Sample No.3 1 Loading Dye(μL) 1 dH2O(μL) 4 TOTAL(μL) 6 - Sample No.3 -> OK ・・・30ng?
- --->Ligation
Sample No. (1) (2) (3) (4) (5) insert①C0178(μL) 1 - - 1 - insert②C0170(μL) - 1 - - 1 vector③J04500(μL) - - 2 2 2 ligase(μL) 1 1 1 1 1 Buffer(μL) 2 2 2 2 2 dH2O(μL) 6 6 5 4 4 TOTAL(μL) 10 10 10 10 10
- --->Transformation
- insert①C0178
- insert②C0170
- vector③J04500
- insert①C0178+vector③J04500
- insert②C0170+vector③J04500
Team:Output
Sample No. ① ② ③ ④ ⑤ DNA tamplate 1 1 1 1 >1 Loading Dye 1 1 1 1 1 dH2O 4 4 4 4 4 TOTAL 6 6 6 6 6 - vector:BBa_F2620
Sample No. BBa_F2620 DNA tamplate 100 SpeⅠ 2 PstⅠ 2 BSA(x10) 13 Buffer2 13 TOTAL 130 - insert:BBa_J52008,BBa_E2030
Sample No. BBa_J52008 BBa_E2030 DNA tamplate 30 30 XbaⅠ 1 1 PstⅠ 1 1 DpnⅠ 1 1 Buffer3 4 4 BSA(x10) 4 4 TOTAL 40 40
-->37℃ 3hour
-->SAP
-->37℃ 30min
-->65℃ 15min
-->Zymo Clean- vector:BBa_F2620 insert:BBa_J52008①
- vector:BBa_F2620 insert:BBa_E2030②
- Negative control:BBa_F2620③
Sample No. ① ② ③ vector 5 5 5 inser 10 7.5 0 Ligase Buffer 4 4 4 Ligase 1 1 1 dH2O 0 2.5 10 TOTAL 20 20 20 -->RT 2hour
-->Transformation(XL10G)
-->37℃ over night
-->colony PCR
-->Mini prep
- --->Zymo Clean