Team:Chiba/Calendar-Home/4 September 2008
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</tr> | </tr> | ||
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- | <td> | + | <td>EcoRI</td><td>0.05</td><td>0.05</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td> | + | <td>PstI</td><td>-</td><td>0.05</td> |
</tr> | </tr> | ||
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:--->(5/9)'''Liquid Culture''' | :--->(5/9)'''Liquid Culture''' | ||
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===Team:Output=== | ===Team:Output=== | ||
[[Team:Chiba/protocol/transformation|Transformation]] | [[Team:Chiba/protocol/transformation|Transformation]] | ||
- | + | :*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x3 | |
- | + | :*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] x3 | |
- | * | + | |
- | * | + |
Latest revision as of 21:04, 29 October 2008
3 September 2008 <|> 5 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- --->Gel Check
|
- --->Digestion test
Sample | Single Digesiton | Double Digestion |
Sample DNA | 1 | 2 |
EcoRI | 0.05 | 0.05 |
PstI | - | 0.05 |
Buffer 3 | 1 | 1 |
BSA | 1 | 1 |
dH2O | 6.95 | 5.9 |
TOTAL | 10μL | 10μL |
- --->Gel Check
|
|
- Transformation
- Competent cells : JW1908
- Transformed the following and grew on new ampicillin plates.
- [http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R-3,4,6,7)
- [http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C-1,2,6,7)
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
- --->(5/9)Liquid Culture
Team:Output
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x3
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] x3