Team:Chiba/Calendar-Home/4 September 2008

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3 September 2008 <|> 5 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

--->Gel Check
Chiba-0904.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007) -> OK
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007) -> OK
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006) -> OK
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006) -> OK
Chiba-0904-2.JPG
From left;
[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2007) -> OK
[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2007) -> OK
[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2006) -> OK
[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2006) -> OK


--->Digestion test
Sample Single DigesitonDouble Digestion
Sample DNA12
EcoRI0.050.05
PstI-0.05
Buffer 3 11
BSA 11
dH2O 6.955.9
TOTAL 10μL10μL


--->Gel Check
Chiba-0904-3.JPG


Sample DNA 10
Loading Dye 2
TOTAL 12μL
From left;
Single Digestion
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
ladder
Double digestion
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
  • [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
  • [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)


Transformation
Competent cells : JW1908
Transformed the following and grew on new ampicillin plates.
  • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R-3,4,6,7)
  • [http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C-1,2,6,7)
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
--->(5/9)Liquid Culture

Team:Output

Transformation

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x3
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] x3