Team:Chiba/jk

From 2008.igem.org

(Difference between revisions)
Line 25: Line 25:
#His TagつきGFP(その他タンパク質)を発現-->freeze-->SDS-PAGE-->blotting
#His TagつきGFP(その他タンパク質)を発現-->freeze-->SDS-PAGE-->blotting
*出力を交換できる形でクローニングしよう
*出力を交換できる形でクローニングしよう
-
 
+
*AHL synthase のkineticsについての情報は？
----
----

Revision as of 16:47, 12 September 2008

>Notebook

August
MTWTFSS
        [http://2008.igem.org/Team:Chiba/Calendar-Main/1_August_2008 1] [http://2008.igem.org/Team:Chiba/Calendar-Main/2_August_2008 2] [http://2008.igem.org/Team:Chiba/Calendar-Main/3_August_2008 3]
[http://2008.igem.org/Team:Chiba/Calendar-Main/4_August_2008 4] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/5_August_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/6_August_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_August_2008&action=edit 7] [http://2008.igem.org/Team:Chiba/Calendar-Main/8_August_2008 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_August_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_August_2008&action=edit 10]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_August_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/12_August_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_August_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_August_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_August_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_August_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_August_2008&action=edit 17]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_August_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/19_August_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/20_August_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_August_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_August_2008&action=edit 22] [http://2008.igem.org/Team:Chiba/Calendar-Main/23_August_2008 23] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/24_August_2008&action=edit 24]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/25_August_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/26_August_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/27_August_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/28_August_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/29_August_2008&action=edit 29] [http://2008.igem.org/Team:Chiba/Calendar-Main/30_August_2008 30] [http://2008.igem.org/Team:Chiba/Calendar-Main/31_August_2008 31]
September
MTWTFSS
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/1_September_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/2_September_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/3_September_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/4_September_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/5_September_2008&action=edit 5] [http://2008.igem.org/Team:Chiba/Calendar-Main/6_September_2008 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/8_September_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_September_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_September_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_September_2008&action=edit 11] [http://2008.igem.org/Team:Chiba/Calendar-Main/12_September_2008 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_September_2008&action=edit 14]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_September_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_September_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_September_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_September_2008&action=edit 18] [http://2008.igem.org/Team:Chiba/Calendar-Main/19_September_2008 19] [http://2008.igem.org/Team:Chiba/Calendar-Main/20_September_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_September_2008&action=edit 21]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_September_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/23_September_2008&action=edit 23] [http://2008.igem.org/Team:Chiba/Calendar-Main/24_September_2008 24] [http://2008.igem.org/Team:Chiba/Calendar-Main/25_September_2008 25] [http://2008.igem.org/Team:Chiba/Calendar-Main/26_September_2008 26] [http://2008.igem.org/Team:Chiba/Calendar-Main/27_September_2008 27] [http://2008.igem.org/Team:Chiba/Calendar-Main/28_September_2008 28]
[http://2008.igem.org/Team:Chiba/Calendar-Main/29_September_2008 29] [http://2008.igem.org/Team:Chiba/Calendar-Main/30_September_2008 30]
October
MTWTFSS
    [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/1_October_2008&action=edit 1] [http://2008.igem.org/Team:Chiba/Calendar-Main/2_October_2008 2] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/3_October_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/4_October_2008&action=edit 4] [http://2008.igem.org/Team:Chiba/Calendar-Main/5_October_2008 5]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/6_October_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_October_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/8_October_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_October_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_October_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_October_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/12_October_2008&action=edit 12]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_October_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/24_October_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/25_October_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/31_October_2008&action=edit 31]
November
MTWTFSS
          [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/1_November_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/2_November_2008&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/3_November_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/4_November_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/5_November_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/6_November_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_November_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/8_November_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_November_2008&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_November_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_November_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/12_November_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_November_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_November_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_November_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_November_2008&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_November_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_November_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/19_November_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/20_November_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_November_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_November_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/23_November_2008&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/24_November_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/25_November_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/26_November_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/27_November_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/28_November_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/29_November_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/30_November_2008&action=edit 30]

To Do List?

  • 出力の決定（杉山、福冨）
  • 入力の決定（香取）
  • Time-delayの確認（井山、川崎、小林、冨永）
-ベクターの乗せ換え(PSB1-->P15A)（井山、小林、川崎）
-cinIのクローニング（井山、川崎）
-BBa_J37015を形質転換(XL10G)-->精製-->ゲルチェック-->形質転換(BW⊿FliC)(冨永)
  • work out deal to print T-shirts (井山、福冨)

still under discussion

  1. 差を広げる-->温度、培地、替えてみる．PFL(Positive feedback loop)(BBa_37015)の利用.
  2. AHL固定(1nM,10nM,100nM~)
  3. 固体培地にspot
  4. His TagつきGFP(その他タンパク質)を発現-->freeze-->SDS-PAGE-->blotting
  • 出力を交換できる形でクローニングしよう
  • AHL synthase のkineticsについての情報は？
入力班 送受信班 出力班 その他

小林あおい Aoi Kobayashi

香取崇広 Takahiro Katori

冨永将大 Masahiro Tominaga

川崎浩平 Kohei Kawasaki

井山佳美 Yoshimi Iyama

福冨浩樹 Hiroki Fukutomi

杉山まい Mai Sugiyama

ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk"