Team:Chiba/jk/β/weak 5

From 2008.igem.org

>送受信班

Contents

Week 5

>last week

14 September, 2008

(13/9)--->Mini prep


Transformation
Competent cells : XL10G
  • BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
  • Plac+RhlI+LVA
  • Plac+CinI+LVA
--->(15/9)

15 September, 2008

Transformation
competent cells : BW⊿FliC
  • BBa_S03623 [http://partsregistry.org/Part:BBa_S03623]
  • BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]
  • BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]
  • BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]
  • BBa_T9002 [http://partsregistry.org/Part:BBa_T9002]

(15/9)->cultured in new LB-Amp medium.
added AHL(total concentration was 0nM,1nM,10nM,100nM,1000nM)

-->check green fluorescence intensity(Excitation:485nm,Emission:527nm)

-->T9002 protocol

Cultured transformants(LB,37℃,14h)

  • BBa_K084007~BBa_K084009
  • BBa_T9002
  • BBa_S03623

cultured above in new LB medium.
-->mix(16/9)

16 September, 2008

(15/9)->mix cultures in the way prescribed in the Table below.

-->check green fluorescence intensity(Excitation:485nm,Emission:527nm)

17 September, 2008

18 September, 2008

19 September, 2008

20 September, 2008

>next week

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