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Team:Freiburg/3D-Modeling
From 2008.igem.org
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== DNA-Origami == | == DNA-Origami == | ||
Planning the input pattern on the DNA-Origami surface, we generated various 3D-models of the huge molecule using "Nano-Engineer", Pymol and SwissPdbViewer (both freeware). Some of those models are shown here to give you an impression of the molecules we have engineered this year.<br> | Planning the input pattern on the DNA-Origami surface, we generated various 3D-models of the huge molecule using "Nano-Engineer", Pymol and SwissPdbViewer (both freeware). Some of those models are shown here to give you an impression of the molecules we have engineered this year.<br> | ||
| - | [[Image:Freiburg2008_Fab_on_Origami_animated.gif|left|thumb|800 px|'''Fig.1:''' Some of the | + | [[Image:Freiburg2008_Fab_on_Origami_animated.gif|left|thumb|800 px|'''Fig.1:''' Some of the oligonucleotides that shape the single-stranded template fused to NIP-molecules and an anti-NIP-Fab-Fragment]] |
<br><br><br> | <br><br><br> | ||
<table> | <table> | ||
<tr> | <tr> | ||
<td> | <td> | ||
| - | [[Image:Freiburg08_DNA-NIP.png|thumb|left|380 px|'''Fig.2:'''DNA-Origami with NIP-molecules and anti-NIP-fragment]] | + | [[Image:Freiburg08_DNA-NIP.png|thumb|left|380 px|'''Fig.2:''' DNA-Origami with NIP-molecules and anti-NIP-fragment]] |
</td> | </td> | ||
<td> | <td> | ||
| - | [[Image:Freiburg08_DNA-NIP5.png|thumb|left|380 px|'''Fig.3:'''Oligo-pattern with scale and two fragments (dimerization model)]] | + | [[Image:Freiburg08_DNA-NIP5.png|thumb|left|380 px|'''Fig.3:''' Oligo-pattern with scale and two fragments (dimerization model)]] |
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| - | [[Image:Freiburg08_DNA-NIP8.png|thumb|left|800 px|'''Fig.4:'''Oligo-pattern with scale and two T-Cell-receptors (dimerization model)]] | + | [[Image:Freiburg08_DNA-NIP8.png|thumb|left|800 px|'''Fig.4:''' Oligo-pattern with scale and two T-Cell-receptors (dimerization model)]] |
</td> | </td> | ||
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| - | [[Image:Freiburg08_DNA-NIP3.png|thumb|left|800 px|'''Fig.5:'''Oligo-pattern with NIP-molecules and Fab-fragment, binding accessibility estimation model]]<br> | + | [[Image:Freiburg08_DNA-NIP3.png|thumb|left|800 px|'''Fig.5:''' Oligo-pattern with NIP-molecules and Fab-fragment, binding accessibility estimation model]]<br> |
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
== Fusion Proteins == | == Fusion Proteins == | ||
| - | The pictures below show models of the fusion proteins our composite parts Bba_K157032 and Bba_K157033 code for (generated with "SwissPdbViewer"):<br> | + | The pictures below show models of the fusion proteins of our composite parts Bba_K157032 and Bba_K157033 code for (generated with "SwissPdbViewer"):<br> |
[[Image:Team-Freiburg2008_Lipo_alpha_nCFP.png|400 px]][[Image:Team-Freiburg2008_Lipo_alpha_link_cCFP.png|400 px]]<br> | [[Image:Team-Freiburg2008_Lipo_alpha_nCFP.png|400 px]][[Image:Team-Freiburg2008_Lipo_alpha_link_cCFP.png|400 px]]<br> | ||
'''Fig.5: Red:''' LipocalinFluA, '''Grey:''' "Transmembrane region (BCR, no structural data; symbolized by helix), '''Blue:''' '''left:''' Split-Cerulean (CFP), N-terminal fragment; '''right:''' Split-Cerulean (CFP), C-terminal fragment<br> | '''Fig.5: Red:''' LipocalinFluA, '''Grey:''' "Transmembrane region (BCR, no structural data; symbolized by helix), '''Blue:''' '''left:''' Split-Cerulean (CFP), N-terminal fragment; '''right:''' Split-Cerulean (CFP), C-terminal fragment<br> | ||
}} | }} | ||
Revision as of 22:04, 29 October 2008
|
_3D-modeling
DNA-OrigamiPlanning the input pattern on the DNA-Origami surface, we generated various 3D-models of the huge molecule using "Nano-Engineer", Pymol and SwissPdbViewer (both freeware). Some of those models are shown here to give you an impression of the molecules we have engineered this year. 
Fusion ProteinsThe pictures below show models of the fusion proteins of our composite parts Bba_K157032 and Bba_K157033 code for (generated with "SwissPdbViewer"): |
