Team:Harvard/Parts/Tempsenseci
From 2008.igem.org
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<div style="text-indent:0pt;color:black">[[Image:122.png|thumb|650px|center|BBa_K098995]]</div> | <div style="text-indent:0pt;color:black">[[Image:122.png|thumb|650px|center|BBa_K098995]]</div> | ||
- | + | [http://partsregistry.org/Part:BBa_K098993 BBa_K098993] is similar, with the strong promoter at the beginning of the part replaced by a weak promoter. | |
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==Induction Test for Thermosensitive cI Systems with GFP Reporters== | ==Induction Test for Thermosensitive cI Systems with GFP Reporters== | ||
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<div style="text-indent:0pt;color:black">[[Image:124.png|thumb|650px|center|BBa_K098988]]</div> | <div style="text-indent:0pt;color:black">[[Image:124.png|thumb|650px|center|BBa_K098988]]</div> | ||
- | + | [http://partsregistry.org/Part:BBa_K098987 BBa_K098987] is equivalent, except that a weak promoter replaces the strong promoter at the beginning of the BBa_K099988. | |
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===Experimental Design=== | ===Experimental Design=== | ||
- | [[Image:Thermo.png|thumb|150px| | + | <div style="text-indent:0pt;color:black">[[Image:Thermo.png|thumb|150px|Thermoinduction Experimental Design]]</div> |
Starter cultures of E. coli with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098988| BBa_K098988] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098987| BBa_K098987] were grown overnight. They were then diluted and grown to OD 0.2 before separation into induced (40 ºC) and uninduced cultures (30 ºC). OD and GFP readings were taken at time 0, 2, and 4 hours. Additionally, after diluting T=2hrs samples to OD 0.2 for accurate GFP measurements, samples were further diluted 1000x, induced (or not induced) again, and placed back in their respective incubators until the end of the experiment, when OD and GFP readings were taken. | Starter cultures of E. coli with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098988| BBa_K098988] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098987| BBa_K098987] were grown overnight. They were then diluted and grown to OD 0.2 before separation into induced (40 ºC) and uninduced cultures (30 ºC). OD and GFP readings were taken at time 0, 2, and 4 hours. Additionally, after diluting T=2hrs samples to OD 0.2 for accurate GFP measurements, samples were further diluted 1000x, induced (or not induced) again, and placed back in their respective incubators until the end of the experiment, when OD and GFP readings were taken. | ||
Latest revision as of 04:58, 30 October 2008
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