"
Team:Hawaii/Notebook/2008-06-12
From 2008.igem.org
(Difference between revisions)
(→Preparation for making competent cells) |
(→Triparental conjugation) |
||
| Line 6: | Line 6: | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | :* Began triparental conjugation between DH5α transformed with pRL1383a, ''E. coli'' with RP1, and PCC6803 [[ | + | :* Began triparental conjugation between DH5α transformed with pRL1383a, ''E. coli'' with RP1, and PCC6803 [[Team:HawaiiTri-parental_conjugation_between_E._coli_and_PCC6803| (see experiment)]] |
::* Innoculated BG-11 + 5% LB plates | ::* Innoculated BG-11 + 5% LB plates | ||
Revision as of 06:44, 13 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Triparental conjugation
- Grace
- Began triparental conjugation between DH5α transformed with pRL1383a, E. coli with RP1, and PCC6803 (see experiment)
- Innoculated BG-11 + 5% LB plates
Purified BBa_B0034 colonies
- Krystle
- Streaked out colonies onto fresh LB+amp100 plates
Preparation for making competent cells
- Krystle & Grace
- Autoclaved detergent-free erlenmeyer flasks
- Innoculated 250 ml SOB w/ DH5α cryostock; shook overnight at 25C with shaking (150 rpm)
Made BG-11+sm+sp plates
- Krystle
- Made 20 plates
- 2.5 μg/ml sm and sp
Made SOB media
- Krystle
- 2 batches
Made BG-11
- Norman
Drylab Work
Sequence synthesis
- Grace
- rbc promoter Biobrick sequence to be synthesized
Parts list
- Norman
- Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper
Discussion
Quote of the Day
- If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
