Team:Hawaii/Notebook/2008-06-12
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:<strong> Grace</strong> | :<strong> Grace</strong> | ||
- | :* Began triparental conjugation between DH5α transformed with pRL1383a, ''E. coli'' with RP1, and PCC6803 [[Tri-parental_conjugation_between_E._coli_and_PCC6803 | (see experiment)]] | + | :* Began triparental conjugation between DH5α transformed with pRL1383a, ''E. coli'' with RP1, and PCC6803 [[Team:Hawaii/Tri-parental_conjugation_between_E._coli_and_PCC6803 | (see experiment)]] |
::* Innoculated BG-11 + 5% LB plates | ::* Innoculated BG-11 + 5% LB plates | ||
Line 15: | Line 15: | ||
=== Preparation for making competent cells=== | === Preparation for making competent cells=== | ||
- | :<strong> Krystle </strong> | + | :<strong> Krystle & Grace </strong> |
:* Autoclaved detergent-free erlenmeyer flasks | :* Autoclaved detergent-free erlenmeyer flasks | ||
+ | :* Innoculated 250 ml SOB w/ DH5α cryostock; shook overnight at 26C with shaking (150 rpm) | ||
=== Made BG-11+sm+sp plates=== | === Made BG-11+sm+sp plates=== | ||
Line 23: | Line 24: | ||
:* Made 20 plates | :* Made 20 plates | ||
- | :* 2.5 μg/ml sm and sp | + | :* 2.5 μg/ml each of sm and sp, labeled Sm<sub>2.5</sub>Sp<sub>2.5</sub> |
=== Made SOB media === | === Made SOB media === | ||
:<strong> Krystle </strong> | :<strong> Krystle </strong> | ||
- | :* 2 batches | + | :* 2 batches (and added antibiotic... oops! will autoclave again to get rid of it) |
=== Made BG-11 === | === Made BG-11 === | ||
:<strong> Norman </strong> | :<strong> Norman </strong> | ||
+ | :* stored in room temp incubator bottom shelf | ||
+ | :* will need to make bunch of 50 mL stock solution of TES and Thisulfate during some downtime. | ||
== Drylab Work == | == Drylab Work == | ||
- | === | + | ===Sequence synthesis=== |
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
:* ''rbc'' promoter Biobrick sequence to be synthesized | :* ''rbc'' promoter Biobrick sequence to be synthesized | ||
- | |||
=== Parts list === | === Parts list === | ||
:<strong> Norman </strong> | :<strong> Norman </strong> | ||
- | |||
:* Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper | :* Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper | ||
+ | |||
+ | === Primer Design Review === | ||
+ | :''' Norman ''' | ||
+ | :* will review some primers tonight before shelling out money to buy'm | ||
== Discussion == | == Discussion == | ||
== Quote of the Day == | == Quote of the Day == | ||
- | * | + | * ''"We like lab math, except when we have to divide by even numbers. We can't divide by even numbers therefore we can't do binary fissions."'' -GK, KS |
- | + | * ''"Bye"'' -GK, KS in synchronized tone... | |
+ | **''"You girls should join a choir..."'' -GP | ||
+ | **''"You not only dress the same, you talk with the same voice at the same time, are you trying to confuse me again!?"'' -NW | ||
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 08:28, 13 June 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Triparental conjugation
- Grace
- Began triparental conjugation between DH5α transformed with pRL1383a, E. coli with RP1, and PCC6803 (see experiment)
- Innoculated BG-11 + 5% LB plates
Purified BBa_B0034 colonies
- Krystle
- Streaked out colonies onto fresh LB+amp100 plates
Preparation for making competent cells
- Krystle & Grace
- Autoclaved detergent-free erlenmeyer flasks
- Innoculated 250 ml SOB w/ DH5α cryostock; shook overnight at 26C with shaking (150 rpm)
Made BG-11+sm+sp plates
- Krystle
- Made 20 plates
- 2.5 μg/ml each of sm and sp, labeled Sm2.5Sp2.5
Made SOB media
- Krystle
- 2 batches (and added antibiotic... oops! will autoclave again to get rid of it)
Made BG-11
- Norman
- stored in room temp incubator bottom shelf
- will need to make bunch of 50 mL stock solution of TES and Thisulfate during some downtime.
Drylab Work
Sequence synthesis
- Grace
- rbc promoter Biobrick sequence to be synthesized
Parts list
- Norman
- Parts list submitted by team members; Norman will send a request to iGEM for parts not on filter paper
Primer Design Review
- Norman
- will review some primers tonight before shelling out money to buy'm
Discussion
Quote of the Day
- "We like lab math, except when we have to divide by even numbers. We can't divide by even numbers therefore we can't do binary fissions." -GK, KS
- "Bye" -GK, KS in synchronized tone...
- "You girls should join a choir..." -GP
- "You not only dress the same, you talk with the same voice at the same time, are you trying to confuse me again!?" -NW
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]