Team:Hawaii/Notebook/2008-06-13

From 2008.igem.org

(Difference between revisions)
(Prepped Cells for Competency)
(Made BG-11 Plates)
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:* Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).
:* Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).
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=== Made BG-11 Plates===
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=== Cell density test===
:<strong> Grace </strong>
:<strong> Grace </strong>
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+
:* Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
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:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
+
:* Made BG-11 plates for retest next week.
===Autoclaved Media===
===Autoclaved Media===

Revision as of 02:08, 14 June 2008

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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Prepped Cells for Glycerol Stock/Seed Stock

Krystle
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. worked on <blah experiment link>, PCR, ran gel

Prepped Cells for Competency

Grace
  • Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
  • Made SOB for new inoculation.
  • Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).

Cell density test

Grace
  • Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
  • Made BG-11 plates for retest next week.

Autoclaved Media

Grace
  • (2) 500 mL batches of SOB
  • (2) 300 mL batches of LB + 1.5% Agar

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

  • We need to make more BG-ll 50x stock solution and TES 50x stock solution

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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