Team:Hawaii/Notebook/2008-06-13

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Things we did today

Wetlab work

Prepped Cells for Glycerol Stock/Seed Stock

Krystle
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. worked on <blah experiment link>, PCR, ran gel

Prepped Cells for Competency

Grace
  • Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
  • Made SOB for new inoculation.
  • Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).

Cell density test

Grace
  • Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
  • Made BG-11 plates for retest next week.

Made LB agar stock

Grace
  • (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C.

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

  • We need to make more BG-ll 50x stock solution and TES 50x stock solution

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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