Team:Hawaii/Notebook/2008-06-13

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Things we did today

Wetlab work

Prepped Cells for Glycerol Stock/Seed Stock

Krystle
  • Inoculated a single colony in each 10 mL LB + antibiotic
Title
Antibiotic Cell type Plasmid
Amp100 StrataClone supercompetent cells pUC18
DH5αGFP
DH5αtet+GFP
DH5αRBS (BBa_B0034)
Kan50HB101RK1
Sm2.5 + Sp 2.5DH5αpRL1383a


Prepped Cells for Competency

Grace
  • Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
  • Made SOB for new inoculation.
  • Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5α. Incubated overnight at 27C with shaking (150 rpm).

Cell density test

Grace
  • Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
  • Made BG-11 plates for retest next week.

Made LB agar stock

Grace
  • (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C.

Discussion

  • We need to make more BG-ll 50x stock solution and TES 50x stock solution
  • Kelly from SC lab will be in 12pm-3pm. We should retrieve our triparental conjugation plates then.
  • Grace, Krystle, and Norman will come in at 9am tomorrow to plate triparental conjugates, make competent cells, and glycerol stock purified Biobrick colonies.

Quote of the Day

E. coli homes. Enough said.


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