Team:Hawaii/Notebook/2008-08- 9

From 2008.igem.org

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(Construction of p+r+g, g+t, J33207+t)
 
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:* Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
:* Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
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::* Centrifuged for 75 min. at 4C after adding KoAc
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::* Centrifuged for 90 min. at 4C after adding KoAc
===Cryostock===
===Cryostock===
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:* Redid RE digest overnight
:* Redid RE digest overnight
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===Made KoAc solution for plasmid preps===
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:<strong>Grace</strong>
===Gel Purification===
===Gel Purification===
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:* Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit
:* Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit
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= Discussion =
 
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= Quote of the Day =
 
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<blockquote>''History is the only laboratory we have in which to test the consequences of thought.'' - Étienne Gilson</blockquote>
 
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{{Team:Hawaii/Footer}}
 
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__NOTOC__
 
= Discussion =
= Discussion =

Latest revision as of 05:28, 13 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)


Things we did today

Wetlab work

Plasmid prep

Grace
  • Plasmid prep of E0240, J32007, I14032 ('05 and '08 distributions), I51020
  • Centrifuged for 90 min. at 4C after adding KoAc

Cryostock

Grace
  • Did not take out 2.4 ml of E. coli before doing plasmid prep (oops). Reinoculated for cryostocking tomorrow.

Construction of p+r+g, g+t, J33207+t

Grace
  • RE digest of nir+rbs, plac+rbs, GFP, B0015, J33207
  • Digested nir+rbs, plac+rbs, J33207 with EcoRI and SpeI
  • Digested GFP, B0015 with Pst1 and XbaI
  • Ran digests on EtBr stained 2% agaorse gel
  • Gel did not run despite using new TAE buffer
  • Redid RE digest overnight

Made KoAc solution for plasmid preps

Grace

Gel Purification

Margaret
  • Ran the large-scale PCR of aadA(BB), aadA(pRL1383a), and P1 lytic region on a gel, cut it out, and purified it using the Qiagen Kit


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]