Team:Hawaii/Notebook/2008-09-15

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Things we did today

Wetlab work

PCR from 9/13

Margaret
Gel of saturday's PCR.
  • Rep, oriV- correct size, so restriction digest with X,P of PCR product
  • omega- correct size, but still has a ladder, so re-digest of plasmid prep with S, X
  • oriT did not show up, but I found a re-digest from 7/24 that I will use for further cloning
  • positive control did not work, not sure if negative control worked or if I did something wrong.


Plasmid prep

Grace
  • Inoculated TB+amp100 with:
  • 3A: nir+rbs+slr1 #2, 3
  • 3A: nir+rbs+pilA #6
  • 3A: nir+rbs+slr1+GFPf #10
  • 3A: nir+rbs+pilA+GFPf12 # 2, 6
  • 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
  • Inoculated TB+sp100
  • BB-pRL1383a

Sequencing

Grace
  • ExoSAP'd, determined DNA conc., prep'd for sequencing:
  • 3A: nir+rbs+slr1 #2, 3
  • 3A: nir+rbs+pilA #6
  • 3A: nir+rbs+slr1+GFPf #10
  • 3A: nir+rbs+pilA+GFPf12 # 2, 6
  • 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
  • plac+rbs+slr1+GFPf #8
  • Sent in samples from today and from last week for sequencing by CORE Hawaii

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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