Team:Hawaii/Notebook/2008-09-16

From 2008.igem.org

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> :* 5 ml plasmid prep of: ::* BB-pRL1383a ::* 3A: nir+rbs+slr1 #2, 3 ::* 3A: n...)
(Wetlab work)
 
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::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
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== Drylab Work ==
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===Gel Purification===
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:<strong>Krystle</strong>
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:* gel purified restriction digest products of:
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:** nir
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:** pilA
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:** slr
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:** pSB1A3
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:** GFPf + tt #8
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:** GFPf + tt #17
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:** GFPf + tt #21
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===Name of Task===
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===Gel Verify constructs===
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:<strong> name of person/people who performed the task</strong>
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-
 
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
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:* e.g. read through papers, worked on proposal, etc.
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[[Image:gel_veri_9_16.jpg|right|thumb|200px|Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).]]
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:<strong>Margaret</strong>
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:*verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
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:*verified constructs will be gel purified and used in a ligation.
= Discussion =
= Discussion =

Latest revision as of 01:43, 21 September 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep

Grace
  • 5 ml plasmid prep of:
  • BB-pRL1383a
  • 3A: nir+rbs+slr1 #2, 3
  • 3A: nir+rbs+pilA #6
  • 3A: nir+rbs+slr1+GFPf #10
  • 3A: nir+rbs+pilA+GFPf12 # 2, 6
  • 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10

Gel Purification

Krystle
  • gel purified restriction digest products of:
    • nir
    • pilA
    • slr
    • pSB1A3
    • GFPf + tt #8
    • GFPf + tt #17
    • GFPf + tt #21

Gel Verify constructs

Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).
Margaret
  • verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
  • verified constructs will be gel purified and used in a ligation.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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