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Team:Hawaii/Notebook/2008-09-16
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep=== :<strong> Grace</strong> :* 5 ml plasmid prep of: ::* BB-pRL1383a ::* 3A: nir+rbs+slr1 #2, 3 ::* 3A: n...) |
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::* 3A: nir+rbs+pilA+GFPf12 # 2, 6 | ::* 3A: nir+rbs+pilA+GFPf12 # 2, 6 | ||
::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10 | ::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10 | ||
| + | |||
| + | ===Gel Verify constructs=== | ||
| + | :<strong>Margaret</strong> | ||
| + | [[Image:gel_veri_9_16.jpg|right|thumb|300px|Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).]] | ||
| + | |||
| + | :*verified constructs: ap36, ab92, ab94, ab95, ab96, ab98 | ||
| + | :*verified constructs will be gel purified and used in a ligation. | ||
| + | |||
== Drylab Work == | == Drylab Work == | ||
Revision as of 19:37, 16 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- 5 ml plasmid prep of:
- BB-pRL1383a
- 3A: nir+rbs+slr1 #2, 3
- 3A: nir+rbs+pilA #6
- 3A: nir+rbs+slr1+GFPf #10
- 3A: nir+rbs+pilA+GFPf12 # 2, 6
- 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
Gel Verify constructs
- Margaret
- verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
- verified constructs will be gel purified and used in a ligation.
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
