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Team:Hawaii/Notebook/2008-09-22
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of transformants=== :<strong> Grace</strong> [[Image:092208colonypcr.png|right|thumb|300px|EtBr stained 2%...) |
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::* nir+rbs+GFP #1, 4, 7 | ::* nir+rbs+GFP #1, 4, 7 | ||
::* nir+rbs+slr1+GFPf #2, 8 | ::* nir+rbs+slr1+GFPf #2, 8 | ||
| - | ::* nir+rbs+pilA # | + | ::* nir+rbs+pilA #18 |
| - | + | :::* Also inoculated using pSB1A3 for 3A vector | |
| - | + | ||
= Discussion = | = Discussion = | ||
Revision as of 02:03, 23 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colonies |
|---|---|
| nir+rbs+pilA | 95 |
| nir+rbs+GFP | 10 |
| nir+rbs+slr1+GFPf | 42 |
| rbs+slr1+GFPf | 2 |
| rbs+GFP+tt | 3 |
| slr1+GFPf+tt | 2 |
| pSB1A3 (to self, negative control) | 330 |
| no DNA | 0 |
- Colony PCR of transformants
- Restreaked and inoculated for plasmid prep:
- rbs+GFPf+tt #1, 2
- nir+rbs+GFP #1, 4, 7
- nir+rbs+slr1+GFPf #2, 8
- nir+rbs+pilA #18
- Also inoculated using pSB1A3 for 3A vector
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
