Team:Hawaii/Notebook/2008-10-20

From 2008.igem.org

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Things we did today

Wetlab work

Large scale plasmid prep of BB-pRL1383a (blue)

Grace

PCR of J04430 (lac+rbs+GFP+tt+tt)

Grace
  • Will act as (-) control for GFP secretion in cyanos

Overnight RE digest

Grace
  • EcoRI and PstI digest of BBpRL1383a and J04430

Made LB+sp100 plates

Grace

Prepped for sequencing

Krystle
  • Sent out correct-sized product to CORE for sequencing
  • nir+rbs
  • slr+gfpf

Construction of GFP secretion device

Krystle
  • Restriction digested additional PCR product
  • nir+rbs with EcoRI and SpeI

Discussion

Quote of the Day

Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)


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