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Team:Hawaii/Notebook/2008-10-29
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic Test, round 2
- Grace
| Plate | Cells | Colonies | Blue? |
|---|---|---|---|
| LB+Amp100X-gal (+) control | J33207 in pSB1A2 in DH5α | too many to count, medium | yes |
| LB+sp100X-gal (+) control | BBpRL1383a-1 (10/25 inoculation plasmid prep) in DH5α | too many to count, small | no |
| LB+X-gal (+) control | untransformed DH5α | lawn/smear | no |
| LB+sp2.5+X-gal (-) control | untransformed DH5α | 0 | no |
| LB+sp100 (-) control | untransformed DH5α | 0 | no |
| LB+sp2.5 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
| LB+sp5 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
| LB+sp10 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
| LB+sp20 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
| LB+sp25 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
| LB+sp40 | J33207+pRL1383a ligation in DH5α | too many to count, small | no |
| LB+sp50 | J33207+pRL1383a ligation in DH5α | too many to count, small to medium | no |
| LB+sp75 | J33207+pRL1383a ligation in DH5α | too many to count, small to medium | no |
| LB+sp100 | J33207+pRL1383a ligation in DH5α | too many to count, small to medium | no |
- Colony PCR of J33207, BB-1, and sp75, 100transformants
Drylab Work
Project page
- Grace
- Updated materials, methods and results for Project Part B.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
