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Team:Hawaii/Protocols
From 2008.igem.org
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* [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]] | * [[Team:Hawaii/Protocols/oligo_hybridization|Oligonucleotide hybridization]] | ||
* [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]] | * [[Team:Hawaii/Procols/DNA_ligation|DNA ligation]] | ||
| - | * DNA restriction digest | + | * [[DNA restriction digest]] |
* [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]] | * [[Team:Hawaii/Protocols/gel_extraction|Gel extraction]] | ||
| + | * [[Team:Hawaii/Protocols/ExoSAP|ExoSAP]] | ||
===''E. coli''=== | ===''E. coli''=== | ||
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* [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]] | * [[Team:Hawaii/Protocols/Competent_Cells | Making competent cells]] | ||
* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | * [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | ||
| - | * Alkaline lysis / plasmid isolation | + | * [[Team:Hawaii/Protocols/Plasmid_prep|Alkaline lysis / plasmid isolation]] |
| + | * [[Team:Hawaii/Protocols/Colony_PCR|Colony PCR]] | ||
=== ''Synechocystis'' sp. PCC6803=== | === ''Synechocystis'' sp. PCC6803=== | ||
Latest revision as of 23:52, 18 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
List of Protocols
This is a list of tried and true protocols used by our team.
In vitro
E. coli
- Cryopreserve cells
- Making competent cells
- Transformation of E. coli
- Alkaline lysis / plasmid isolation
- Colony PCR
Synechocystis sp. PCC6803
Media
- Solid growth media
- Liquid growth media
- Antibiotics
BioBrick
- [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
- [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
- [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
- [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
