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Team:KULeuven/10 September 2008
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== Lab Work == | == Lab Work == | ||
=== Wet Lab === | === Wet Lab === | ||
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| + | * We MiniPrepped the ligations that succeeded yesterday: K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015. Then K145258 was cut with ''EcoR''I and ''Spe''I. | ||
| + | * We also made a glycerolstock of these ligations. | ||
| + | * The digests we made yesterday were put on gel. Then we extracted them and put again 2ul on gel to estimate the concentration. | ||
| + | * We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2). | ||
| + | * We cut B0034 and J23109+J23032 with ''Eco''RI and ''Xba''I. | ||
| + | * We made a liquid culture of K145279 and K145280 to test the relation input/output with anhydrotetracycline. | ||
=== Dry Lab === | === Dry Lab === | ||
Blending the ethics... | Blending the ethics... | ||
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== Remarks == | == Remarks == | ||
FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!! | FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!! | ||
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Latest revision as of 01:45, 30 October 2008
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Contents |
Lab Work
Wet Lab
- We MiniPrepped the ligations that succeeded yesterday: K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015. Then K145258 was cut with EcoRI and SpeI.
- We also made a glycerolstock of these ligations.
- The digests we made yesterday were put on gel. Then we extracted them and put again 2ul on gel to estimate the concentration.
- We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2).
- We cut B0034 and J23109+J23032 with EcoRI and XbaI.
- We made a liquid culture of K145279 and K145280 to test the relation input/output with anhydrotetracycline.
Dry Lab
Blending the ethics...
Remarks
FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!!
