Team:KULeuven/18 August 2008

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Contents

Lab Work

Wet Lab

  • We did some PCRs to prepare the following (new) BioBricks: K145014 (T7 polymerase with UmuD), K145013 (antisense LuxI) and K145151 (ccdB).
  • We made a few digests: F1610 and B0015 were cut with EcoRI and XbaI, and J23100+B0032 and R0062+B0032 were cut with EcoRI and SpeI.
  • We electroporated the following ligated parts: C0040+B0015, K145015+B0015, C0012+B0015 and C0060+B0015.
  • We continued ligating: J23116+B0032, R0040+B0032, R0040+J23022, K145001+pSB1A2, K145001+B0015, R0084+B0032 and (R0084+J23022)+(J23109+J23032). this time, B0032 was first dephosphorylated.

Dry Lab

Modeling

Association and dissociation rates have been reinvestigated (HSL with LuxR), wrong values have been removed, LuxR autoactivation has been further incorporated; we've also included diffusion of HSL.

Wiki

Small updates for the wiki: adjusting pictograms, home page, ...

Started defining a global css, intermediate result here: Team:KULeuven/Zandbak/Global

Remarks

TGS2.PNG

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