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Team:KULeuven/19 September 2008
From 2008.igem.org
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With these digests we could start some new ligations, they will be electroporated by one of our advisors next week: | With these digests we could start some new ligations, they will be electroporated by one of our advisors next week: | ||
| - | <div style="margin-left:100px;">(J23116+B0034)+(C0040+B0015) = [http://partsregistry.org/Part: | + | <div style="margin-left:100px;">(J23116+B0034)+(C0040+B0015) = [http://partsregistry.org/Part:BBa_K145201 K145201]</div> <div style="margin-left:100px;">(K145201)+(K145205) = [http://partsregistry.org/Part:BBa_K145280 K145280]</div> <div style="margin-left:100px;">(K145201)+(R0040+E0240) = [http://partsregistry.org/Part:BBa_K145279 K145279]</div> <div style="margin-left:100px;">(J23116+B0034)+(C0062+B0015) = [http://partsregistry.org/Part:BBa_K145270 K145270]</div> |
Latest revision as of 17:54, 29 October 2008
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Lab Work
Wet Lab
We did one last measurement of the fluorescence of GFP and GFP-LVA. After that, we started the data analysis. In each FACS measurement the fluorescence of 10,000 cells was measured. So, we calculated the mean and standard deviation (SD) of the fluorescence of all the cells in each measurement. The rest of the data analysis will be done tomorrow.
We made some new digests of the following parts (half of them failed though):
K145201 -> cut with EcoR I and Spe I (succeeded).
K145275 -> cut with EcoR I and Spe I (failed).
With these digests we could start some new ligations, they will be electroporated by one of our advisors next week:
(J23116+B0034)+(C0040+B0015) = K145201
(K145201)+(K145205) = K145280
(K145201)+(R0040+E0240) = K145279
(J23116+B0034)+(C0062+B0015) = K145270
