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Team:KULeuven/1 September 2008
From 2008.igem.org
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== Lab Work == | == Lab Work == | ||
=== Wet Lab === | === Wet Lab === | ||
| - | + | We did a lot of PCRs today. We tried to add the second part of the UmuD tag with the 91bp-primer. We had very sharp and extremely beautiful bands, but they seemed three times too long: FAIL. This will be done again tomorrow, with primers 2.1 and 2.2. The antisense LuxI PCR resulted in a smear: FAIL again. To be done again tomorrow. We also did a PCR to test the ligations. | |
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| - | + | We miniprepped the ligations we streaked out yesterday and part [http://partsregistry.org/Part:BBa_R1052 R1052] and [http://partsregistry.org/Part:BBa_R0040 R0040]. It's the second time we miniprep R1052 and the concentrations was two times very low. The registry shows that there are a few problems with this part. | |
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| - | + | We made some digests: [http://partsregistry.org/Part:BBa_C0056 C0056] and [http://partsregistry.org/Part:BBa_C0061 C0061] with ''Spe''I and ''Pst''I and [http://partsregistry.org/Part:BBa_R0040 R0040] with ''Spe''I and ''Eco''RI. We couldn't see R0040 on gel and C0056 seemed to be too big, only C0061 was ok. We purified C0061 and C0056. | |
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| + | We made a ligation of [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] (cut with ''Pst''I instead of ''Eco''RI). | ||
=== Dry Lab === | === Dry Lab === | ||
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==== Wiki ==== | ==== Wiki ==== | ||
Components section has been updated further. Also the modeling section is getting some new lines and graphics ... | Components section has been updated further. Also the modeling section is getting some new lines and graphics ... | ||
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Latest revision as of 13:06, 16 October 2008
| << return to notebook | return to homepage >> | ||
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Contents |
Lab Work
Wet Lab
We did a lot of PCRs today. We tried to add the second part of the UmuD tag with the 91bp-primer. We had very sharp and extremely beautiful bands, but they seemed three times too long: FAIL. This will be done again tomorrow, with primers 2.1 and 2.2. The antisense LuxI PCR resulted in a smear: FAIL again. To be done again tomorrow. We also did a PCR to test the ligations.
We miniprepped the ligations we streaked out yesterday and part R1052 and R0040. It's the second time we miniprep R1052 and the concentrations was two times very low. The registry shows that there are a few problems with this part.
We made some digests: C0056 and C0061 with SpeI and PstI and R0040 with SpeI and EcoRI. We couldn't see R0040 on gel and C0056 seemed to be too big, only C0061 was ok. We purified C0061 and C0056.
We made a ligation of C0061+B0015 (cut with PstI instead of EcoRI).
Dry Lab
Ethics...
Modeling
Latex-tool... Multi-cell...
Wiki
Components section has been updated further. Also the modeling section is getting some new lines and graphics ...
