Team:KULeuven/24 September 2008

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Lab Work

Dry Lab

We sent some samples out last week to be sequenced. The results arrived today. We've compared these sequences with the sequences that we expect. This gave mixed results.

The following parts are shown to be good:

    • GFP-LVA: [http://partsregistry.org/Part:BBa_K145015 K145015]
    • ccdB gene: [http://partsregistry.org/Part:BBa_K145151 K145151]
    • T7 RNA polymerase (no tag): [http://partsregistry.org/Part:BBa_K145001 K145001]
    • T7 RNA polymerase with UmuD tag: [http://partsregistry.org/Part:BBa_K145014 K145014]
    • antisense LuxI: [http://partsregistry.org/Part:BBa_K145013 K145013]
    • R0040+J23066 (Filter - part 1): [http://partsregistry.org/Part:BBa_K145215 K145215]
    • Output: [http://partsregistry.org/Part:BBa_K145205 K145205]
    • R0040+P0353 (test HPr): [http://partsregistry.org/Part:BBa_K145273 K145273]
    • InverTimer - part 2: [http://partsregistry.org/Part:BBa_K145254 K145254]

The following parts are not correct:

    • [http://partsregistry.org/Part:BBa_K145201 K145201], [http://partsregistry.org/Part:BBa_K145279 K145279] and [http://partsregistry.org/Part:BBa_K145280 K145280]: Input and tests, constructs has no promoter
    • [http://partsregistry.org/Part:BBa_K145253 K145253], [http://partsregistry.org/Part:BBa_K145104 K145104], [http://partsregistry.org/Part:BBa_K145272 K145272] and [http://partsregistry.org/Part:BBa_K145251 K145251]: Reset and InverTimer, constructs contain wrong promoter
    • [http://partsregistry.org/Part:BBa_K145258 K145258] (R0040+B0033+C0056+B0015): construct does not contain R0040 or B0033
    • [http://partsregistry.org/Part:BBa_K145278 K145278] (J23109+J23032+K145001+B0015): Filter, construct does not contain K145001

The following parts were not properly sequenced (inconclusive):

    • Hybrid Promoter: [http://partsregistry.org/Part:BBa_K145150 K145150]
    • test HPr: [http://partsregistry.org/Part:BBa_K145269 K145269]
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