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Team:KULeuven/28 August 2008
From 2008.igem.org
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== Lab Work == | == Lab Work == | ||
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| + | [[image:gel-28-8a.jpg|right|thumb|350px|'''Gel 1''': Test ligation with PCR. All 4 colonies of K145151+pSB1A2 ligated properly, but only the first colony of K145001+pSB1A2 ligated properly]] | ||
| + | [[image:gel-28-8b.jpg|right|thumb|350px|'''Gel 2''': Digests of B0015, C0012, C0060, K145015 and C0040]] | ||
=== Wet Lab === | === Wet Lab === | ||
| - | + | The ligations with B0015 ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]) were electroporated again, but now they were plated on an ampicillin plate. | |
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| - | + | A glycerol stock was made of the succeeded ligations. | |
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| - | + | We ordered some new parts, because the modeling showed that a lot of things had to be changed. They arrived today and they were also plated. | |
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| + | The ligations of [http://partsregistry.org/Part:BBa_K145001 K145001] and [http://partsregistry.org/Part:BBa_K145151 K145151] with [http://partsregistry.org/Part:pSB1A2 pSB1A2] were miniprepped and then we did a PCR to test the ligation (primers VF/VR-2). See '''Gel 1'''. | ||
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| + | The digests of [http://partsregistry.org/Part:BBa_R0011 R0011], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_C0062 C0062] and [http://partsregistry.org/Part:BBa_B0033 B0033] were put on gel and purified. | ||
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| + | Some more digests were made, but this time we cut with ''Spe''I, ''Xba''I and ''Pst''I. We cut [http://partsregistry.org/Part:BBa_B0015 B0015] as insert (''Xba''I and ''Pst''I) and [http://partsregistry.org/Part:BBa_C0060 C0060], [http://partsregistry.org/Part:BBa_K145015 K145015], [http://partsregistry.org/Part:BBa_C0012 C0012] and [http://partsregistry.org/Part:BBa_C0040 C0040] were cut as vector (''Spe''I and ''Pst''I). See '''Gel 2'''. | ||
=== Dry Lab === | === Dry Lab === | ||
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| + | ==== Ethics ==== | ||
We did some more research on bioethics and human practice. | We did some more research on bioethics and human practice. | ||
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Simulations of the different parts has continued. | Simulations of the different parts has continued. | ||
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Latest revision as of 12:18, 12 October 2008
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Contents |
Lab Work
Wet Lab
The ligations with B0015 (C0040+B0015, C0060+B0015, C0012+B0015, K145015+B0015, K145001+B0015, K145151+B0015) were electroporated again, but now they were plated on an ampicillin plate.
A glycerol stock was made of the succeeded ligations.
We ordered some new parts, because the modeling showed that a lot of things had to be changed. They arrived today and they were also plated.
The ligations of K145001 and K145151 with pSB1A2 were miniprepped and then we did a PCR to test the ligation (primers VF/VR-2). See Gel 1.
The digests of R0011, R0040+J23022, C0062 and B0033 were put on gel and purified.
Some more digests were made, but this time we cut with SpeI, XbaI and PstI. We cut B0015 as insert (XbaI and PstI) and C0060, K145015, C0012 and C0040 were cut as vector (SpeI and PstI). See Gel 2.
Dry Lab
Ethics
We did some more research on bioethics and human practice.
Modeling
Great Succes: we succeeded in modeling cell-multiplication in a Matlab-environment. This will be our starting point for modeling cell division and work in a multi-cell environment! [we did what the lab couldn't do]
Parameter checking has almost reached its final state.
Simulations of the different parts has continued.
