Team:KULeuven/2 September 2008

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=== Wet Lab ===
=== Wet Lab ===
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* We tried to construct our parts K145150 (hybrid promoter) and K145014 (T7 polymerase with UmuD tag) using PCR. Both of them failed, but K145150 gave two bands. This is propably because we didn't purified it at first. We will do that tomorrow.
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* We tried to construct our parts K145150 (hybrid promoter) and K145014 (T7 polymerase with UmuD tag) using PCR. Both of them failed, but K145150 gave two bands. This is propably because we didn't purify it at first. We will do that tomorrow.
* We made electrocompetent cells of the JM109 stem.
* We made electrocompetent cells of the JM109 stem.
* We did some more digests:
* We did some more digests:

Revision as of 18:12, 2 September 2008

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Contents

Lab Work

Wet Lab

  • We tried to construct our parts K145150 (hybrid promoter) and K145014 (T7 polymerase with UmuD tag) using PCR. Both of them failed, but K145150 gave two bands. This is propably because we didn't purify it at first. We will do that tomorrow.
  • We made electrocompetent cells of the JM109 stem.
  • We did some more digests:
cut with SpeI and EcoRI -> R0040, B0014, R0053 and R1052 (R1052 failed).
cut with XbaI -> K145151+B0015, C0040+B0015, C0060+B0015, C0012+B0015 and K145015+B0015.
  • We started a few ligations: R0040+B0033, R0053+P0152, R0040+J23066, B0014+B0033 and R0040+E0240.
  • We also tested the ligations of 31 August using PCR. Most of them were OK. The ligations that succeeded so far can be found on the schemes below.
Current Status (Final Scheme):
2sept final.PNG
Current Status (Parallel Scheme):
2sept parallel.PNG

Dry Lab

Ethics...

Modeling

Latex-tool... Multi-cell... Diffusion... Updating wiki...

Wiki

Remarks

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