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Team:KULeuven/6 September 2008
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(New page: {{:Team:KULeuven/Tools/Header}} == Lab Work == === Wet Lab === Busy day for a saturday ;) * Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy...) |
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* Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts. | * Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts. | ||
* PCR with prefix-sufix primers on R1052 and the hybrid promoter. Gave some weird results. Will need to be looked at again. | * PCR with prefix-sufix primers on R1052 and the hybrid promoter. Gave some weird results. Will need to be looked at again. | ||
| + | * Made a couple digests: R0040+E0240, C0061+B0015 and C0056+B0015 were cut with ''Eco''RI, E0040 was cut with ''Spe''I and ''Pst''I, and B0015 was cut with ''Xba''I and ''Pst''I. They were put on gel and then purified. The results looked very good. | ||
| + | * Also made a digest of K145201, K145205, K145253, K145251 and R0040+J23022+J23109+J23032, but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-) | ||
| + | * Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015. | ||
| + | * We set up a PCR to test our ligations using the VF2/VR primers. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 18:03, 6 September 2008
dock/undock dropdown
Contents |
Lab Work
Wet Lab
Busy day for a saturday ;)
- Made some new electrocompetent TOP10 cells, we've got about 32 new aliquots of bacteria happy to be fried by a thousand volts.
- PCR with prefix-sufix primers on R1052 and the hybrid promoter. Gave some weird results. Will need to be looked at again.
- Made a couple digests: R0040+E0240, C0061+B0015 and C0056+B0015 were cut with EcoRI, E0040 was cut with SpeI and PstI, and B0015 was cut with XbaI and PstI. They were put on gel and then purified. The results looked very good.
- Also made a digest of K145201, K145205, K145253, K145251 and R0040+J23022+J23109+J23032, but then I forgot to load the Smartladder onto the gel. These digest were set up again (overnight reaction). Anyway, the gel already looked very promising ;-)
- Started some new ligations (mainly to test the new hybrid promoter): R0040+P0353, (R0040+B0032)+(C0062+B0015), (R0011+B0032)+(C0061+B0015) and E0040+B0015.
- We set up a PCR to test our ligations using the VF2/VR primers.
Dry Lab
Modeling
Wiki
Remarks
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